LOCUS       sequence-68753-e         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A retroviral vector for the constitutive expression of soluble 
            guanylate cyclase1 beta3 double mutant (E473K and C541D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T
  TITLE     Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that 
            increases endothelial tau phosphorylation and permeability.
  JOURNAL   J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 
            10.1074/jbc.M111.301440. Epub 2012 May 25.
  PUBMED    22637478
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.301440"; journalName: "J Biol Chem"; date: 
            "2012-07-20- 20"; volume: "287"; issue: "30"; pages: "25407-18"
COMMENT     Sequence Label: pMA3411
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     70..86
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     70..86
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ccgtcgacct cgaggggggg cccggtaccg aggacgcgtc aattcgagct ccaccgcggt
       61 ggcggccgct ctagaactag tggatccccc gggctgcagg aattcgatct ctagattcag
      121 ttttcatcct ggtttgtttc ctctgtgcct gtgtttttcc tggatagaaa ccagacttgc
      181 attggctcct tcttgccctt catggacaca ggacctctgt gctccaagtg gaactgtgga
      241 tccgagtttt ctggggacat gagacacctg tatgtatatt cggaaacgtt tatcttcccc
      301 ttttctcctg tggtttctgt cctgcttgtg aggttgacag tatttccgaa gagatcatac
      361 cgtggcatcc gctgtccaat cacacctgtc accacctccc cagtgtggat cccgatggtt
      421 atctgaacag attcgccatc tacttgaact tgaccagcaa tttccatcat gtctaaagcc
      481 aggtggcaaa tggaccgtgc atggtggata caaggttctg gcaaaccact cactgtcata
      541 tacttgtcac caactgtttt caccttgtaa acaaatgggt ttttccgtga at
//
