LOCUS       sequence-68754-e         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector, Hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T
  TITLE     Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that 
            increases endothelial tau phosphorylation and permeability.
  JOURNAL   J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 
            10.1074/jbc.M111.301440. Epub 2012 May 25.
  PUBMED    22637478
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.301440"; journalName: "J Biol Chem"; date: 
            "2012-07-20- 20"; volume: "287"; issue: "30"; pages: "25407-18"
COMMENT     Sequence Label: pMA1662
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..60)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(44..60)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     117..133
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     118..134
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(156..176)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        161..490
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      341..476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 attcgccagc acagtggtcg aatcgaattc ctgcagcccg ggggatccac tagttctaga
       61 gcggccgcca ccgcggtgga gctcgaattg acgcgtcctc ggtaccgggc cccccctcga
      121 ggtcgacggt atcgataagc ttgattcgac cctgtggaat gtgtgtcagt tagggtgtgg
      181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      241 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      301 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      361 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      421 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      481 cttttgcaaa aagctagctt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
      541 agatctgatc ccggggggca atgagatatg aaaaagcct
//
