LOCUS       sequence-68755-e         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for Tet-inducible expression. 
            Puromycin-resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T
  TITLE     Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that 
            increases endothelial tau phosphorylation and permeability.
  JOURNAL   J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 
            10.1074/jbc.M111.301440. Epub 2012 May 25.
  PUBMED    22637478
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.301440"; journalName: "J Biol Chem"; date: 
            "2012-07-20- 20"; volume: "287"; issue: "30"; pages: "25407-18"
COMMENT     Sequence Label: pMA3211
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      4..139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9..306)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     304..324
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(373..389)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..389)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     446..462
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     447..463
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(491..515)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
       61 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
      121 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
      181 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
      241 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
      301 cacaccctaa ctgacacaca ttccacaggg tcctccaccg cggtggcggc catcaattcc
      361 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctcgaattga
      421 cgcgtcctcg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa
      481 ttctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta
      541 cac
//
