LOCUS       sequence-68757-e        8697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length ASP ectodomain 
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: ASP-bio
FEATURES             Location/Qualifiers
     source          1..8697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4077..4132
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4131..4150)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6368..6386)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6454..6558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6559..6627,6628..7419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6777..6796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7590..8178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8079..8098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8332..8349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8466..8487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8502..8507,8508..8525,8526..8532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8540..8556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8545..8567
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8564..8580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8564..8580
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaaag agaacaagaa cgacaacttc ctgaacggca
     1381 tctacaagct gcagaacatc tggcggaaca ccgccctggg cgagctggac ctgaagaagg
     1441 ccaacatcga gaacgccgag ggcatcatcg agaagttcaa cgacgctaac aaggctgact
     1501 tcaaccagca cccccacttc agaagcaagc tgtccttcct ggaacacaag aaccacaagg
     1561 acatgggcat cgtgaccaac caccagaaca aggaccacaa gaagaacgag tacaccagcg
     1621 aagagatcat cgacatgaag ccccagctgg catctcagga aatgtggcgg aagggcgaga
     1681 acttctacat gcacggcgcc accaacacca acgagcagaa cgacgacgag gacctgttcg
     1741 agagcttcca caagaatcac aacagcaaca tcaagatcaa cagcccctgc ttcgatctgc
     1801 aagacaaaga gatgtgcagc aacaacaaag agtgcttcta cgacgacgcc tacaaagcct
     1861 gcttccagaa ctgcgccctg gtcaacgaga agaagtgcag cctgtacagc gagtgcaagc
     1921 acacccccaa cggctgccag aacgagggct acctgaacat ccaggtgttc ggcagcaatc
     1981 tgggcggaga tgtgcgggcc tgcgagctgt ttgagagcga gggcagctgc tacctgatgg
     2041 aaaagctgtt caagaagctg tccgaggaaa agaaagagac aagcttcaac tgcgtgtgga
     2101 tcagctacaa gcacgagctg cggagctacg acgatgagaa cgagcccaaa gagaataacg
     2161 acaacatcgt ggccctggaa aataagaaca agtacatgta catgaacaac gtggagagca
     2221 tccacaacaa ccaccacctg gaaaacccct accacgagga catcaagcac aataagcaca
     2281 agagcttcaa ctaccacgtg ctgtccagca gcgtgccccg gcagatcgcc aagaacgaga
     2341 agtttctgag cctgctgcag ctgaacctga agggcaagaa gaaagagaag aacaagaaga
     2401 agaaaaagga cgacgaagat gacgacgaca tcgacgaaga ggatgacgat ctggacatcg
     2461 agggcaagaa caacgagttc aacgccgacg aggaagagaa cagcaaggat ctgaagaaca
     2521 acaacatcga cgacaacgaa gaggacggca ccaccgacga ggacgacctg tttaacaaca
     2581 agaaaaaaaa gaagaataac aataacaaca atgccaacaa caataataac aagaacaaaa
     2641 acaagcagaa caccaataag aaaaacaaca agatcatcaa cgagttcgac gacgatatcg
     2701 atgaggtgtc cgaagagaat ctgctgaacg acgaagagaa gaagcagaac aagatcaaga
     2761 ataagaacat caatgaggcc aacaatatcg ccgagaacaa tgagatcgag atcagcagca
     2821 aggacctgac cgagcagtac gaggacaagt acaactacga caaaaagaac aacatgggca
     2881 ccaacaacaa cttcaacaac gccaatacct tcgccaacaa taagaataac ctccacaata
     2941 agtacatcaa ccacgccagc cacacccaga atgagaagga catcctgaag cacaacttct
     3001 acggcaagga caacaacggc gcctacgaga tcatcaaggt ggagacacac atctgcgcca
     3061 acctgaacga gaagcccaac cccagcacac tgctggaagg cgccctgatc gccgaaaacg
     3121 aggcccagct gcagaagatc aaaaagaagt acaaagtcac cgacaacgag atctgcgtgc
     3181 ggcccaccaa tagcgcctac gtgtccctga tccccgacaa gaagtactat ctgctgggcg
     3241 agaagatcga gttcaagtgc caggaaggct acaagatcat tggcaccacc aacatcggcg
     3301 tgtgcaccgg cagaaacatc atcagcccca atatcgcctg cgagagcctg accaacttcg
     3361 acgacgtgga gaaggacaat atccagaaga tgaacaacat catcaattct ggcgcgccgt
     3421 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     3481 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     3541 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     3601 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     3661 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     3721 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     3781 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     3841 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     3901 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     3961 aagggttgaa ttccggatca ctgcatcata ttctggatgc acagaaaatg gtgtggaatc
     4021 atcgttaagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     4081 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     4141 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     4201 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     4261 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     4321 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     4381 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     4441 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     4501 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     4561 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     4621 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     4681 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     4741 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     4801 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     4861 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     4921 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     4981 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     5041 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     5101 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     5161 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     5221 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     5281 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     5341 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     5401 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     5461 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     5521 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     5581 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     5641 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     5701 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     5761 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     5821 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5881 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5941 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     6001 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     6061 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     6121 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6181 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     6241 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     6301 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     6361 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6421 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6781 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     6841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7081 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     7141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7441 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8221 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8281 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8341 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8401 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8461 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8521 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8581 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     8641 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatgga
//
