LOCUS       sequence-68758-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for the dual control of expression through Tet-On 
            promoter and C-terminal destabilizing domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T
  TITLE     Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that 
            increases endothelial tau phosphorylation and permeability.
  JOURNAL   J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 
            10.1074/jbc.M111.301440. Epub 2012 May 25.
  PUBMED    22637478
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.301440"; journalName: "J Biol Chem"; date: 
            "2012-07-20- 20"; volume: "287"; issue: "30"; pages: "25407-18"
COMMENT     Sequence Label: pMA3174
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(8..24)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9..25)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             100..129
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             130..453
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     primer_bind     complement(495..515)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agcttatcga taccgtcgac ctcgaggggg ggcccggtac cgaggacgcg tcaattcgag
       61 ctccaccgcg gtggcggccg ctctagaact agtcccgggg agcagaaact catcagcgag
      121 gaggacctgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
      181 cgcggccaga cctgtgtggt gcactacacc gggatgcttg gagatggaaa gaaagtcgat
      241 tcctctcggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
      301 ggctgggaag aaggggttgc ccagatgagt gtgggtcagg gagccaaact gactatatct
      361 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
      421 gtcttcgatg tggagcttct ggaactggaa taaatgcagg aattgatggc cgccaccgcg
      481 gtggaggacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      541 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      721 ccatggctga ctaatttttt ttatttatgc
//
