LOCUS       sequence-68760-e        7863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MTRAP 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MTRAP-bio
FEATURES             Location/Qualifiers
     source          1..7863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3243..3298
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3297..3316)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5534..5552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5620..5724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5725..5793,5794..6585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5943..5962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6756..7344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7245..7264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7498..7515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7632..7653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7668..7673,7674..7691,7692..7698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7706..7722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7711..7733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7730..7746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7730..7746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccatca gcacccacga cacctgtgac gagtggagcg
     1381 agtggtccgc ctgtacccac ggcatcagca ccagaaagtg cctgagcgac agcagcatca
     1441 aggacgagac actcgtgtgc accaagtgcg acaagtgggg ggagtggtcc gagtgcaagg
     1501 acggccggat gcaccggaag gtgctgaact gccccttcat caaagaggaa caggaatgcg
     1561 acgtcaacaa cgagatggcc gaggacaccc acatgaacaa cgcctacatc tacttcaacg
     1621 ccgacgacgg cgacaacgag tacgaggacc acgacgacaa gaacgacgac gataagaatt
     1681 acgacaacga gaatgacgat gacaagaatg atgatgacaa aaacgatgat gacaaaaacg
     1741 acgacgacaa aaatgatgat gacaagaatg acgatgagga aaactacaac gacgccgagg
     1801 aaaaagtgaa gaacaacgac atccacaaca gcgccgccaa cagcaacaac gaagtgacca
     1861 acttcatcca gatcaaggac aagatcatga tcaagcacaa gaccaccaac atccaccccg
     1921 tgaactttat ccaggaaaag tacacccgga acaacaagta cagaagcgac aacttcgcca
     1981 agatcctgaa caacatgaac cacatcaaca acaacaacta caacagccgg tccagcagca
     2041 ccagctccaa gaacgccaga ggctacagag gcggcagcag caacatgtac ccccacgtgc
     2101 ccaactacgc cagcagcagc gtgcacaact ccgccaacaa cgagcggaag tccgacgagg
     2161 acctggacaa catcgagggc gacaatatcg ccaaagagga aagaatcgtc cccatcaata
     2221 acaagaacta tgacaaccac gacgagcaca gcaacatcca cgagcacgac accagccgga
     2281 acgtggacaa tgagaagtac aactccaacg acgatctgcc caacctggcc acctacgact
     2341 acgacatgaa caatgacgcc tacaagaaaa accacatgaa gaaacccatg gacagcatta
     2401 aagaagaaca gaccaagcag gaaaacaacc agaacaacga gaaggtgtcc agcagcgaga
     2461 agcagaacga cgatatcagc gccctgtacg agcacatgaa caccaaggac caggaacaca
     2521 cccagcacga gcagcccaat gatgccgccc acggccactt cgaggactac agcaagggcg
     2581 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2641 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     2701 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     2761 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     2821 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     2881 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     2941 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     3001 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     3061 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     3121 tatccaaagg gttgaattcc ggatcactgc atcatattct ggatgcacag aaaatggtgt
     3181 ggaatcatcg ttaagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg
     3241 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     3301 cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag
     3361 aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
     3421 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
     3481 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
     3541 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
     3601 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     3661 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     3721 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     3781 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
     3841 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
     3901 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
     3961 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
     4021 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
     4081 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
     4141 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
     4201 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
     4261 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
     4321 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
     4381 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     4441 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     4501 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     4561 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     4621 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     4681 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     4741 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     4801 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     4861 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     4921 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     4981 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     5041 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     5101 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     5161 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     5221 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     5281 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     5341 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     5401 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     5461 atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct
     5521 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5581 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5641 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5701 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5761 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5821 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5881 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5941 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     6001 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6061 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6121 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6181 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6241 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     6301 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6361 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6421 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6481 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6541 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6601 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6661 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6721 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6781 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6841 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6901 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6961 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7021 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7081 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7141 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7201 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7261 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7321 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7381 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7441 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7501 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7561 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7621 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7681 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7741 tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct
     7801 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     7861 gga
//
