LOCUS       sequence-68762-e       14322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length EBL1 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14322)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 14322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: EBL1-bio
FEATURES             Location/Qualifiers
     source          1..14322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9702..9757
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9756..9775)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11993..12011)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12079..12183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12184..12252,12253..13044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12402..12421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13215..13803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13704..13723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13957..13974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14091..14112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14127..14132,14133..14150,14151..14157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14165..14181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14170..14192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14189..14205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14189..14205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaagg atcagaccag cgagaaagtg aagtcccaga
     1381 aagagaacga cgccctccag gtgcacaaga agaagtccgg cgagctgaac aacaacaagg
     1441 ccggcatcct gcggagcacc tacaagcgga tcgtgggcta cacacagaag aagctggaag
     1501 atctgttcca cgtgaacggc gagtacatca agaaagaaga ggacgagaaa gaaaaggtgg
     1561 accagaacaa ggacatgctg aagtccggcc tgatgatcat cgaccagaat aaggatggcg
     1621 aggaactgga aaacctgatc gaccccatca agaacatcaa ggacagcaac aacctggaaa
     1681 actactacga cgacaactac aacaacatca agttcgacgt gggcaacaag gccaaccacc
     1741 tgaagtacat gaaaacccgg aacagcgaca gcgacgagcg gttcagcaac tactacttca
     1801 tcgacaacaa cctgtttctg cgggacaaca aagagcagga cgagaacgac ttcgagaaga
     1861 acaagttcat cgacacctac agcctgtacg agtgcggcaa gaaaatcaaa gaaatgaagt
     1921 ggatctgcac cgacaaccag ttcaagagca acaatctgtg cgcccccatc agacggatcc
     1981 agctgtgcat cgtgaacatc atcctgttca gcgagaacga gaacgagtac atctacaaga
     2041 acgatgccat caacaacaag ttcaaagaga acatcctgaa ggccgtgaag ctggaaagca
     2101 atctgctggt gcagaagcac aacaacgagt acaacagcaa gctgtgcgac gacatccggt
     2161 ggagctttct ggactacggc gacatcatca tcggccggga cctgatctat aagaacaacg
     2221 ccgactacat caaagagcag ttcaagaaga tcttcaataa cgagtataac aacaatgagc
     2281 tgaacgacga gctgaataac gaactgaacg atgagaagaa tatcaagctg cggaaagagt
     2341 ggtgggagaa gtacaaagag gacatctggg aggaaatgac caaagagcac aacgacaagt
     2401 ttatcgagaa gtgcaagtac ttcgccaagg acgagcccca gatcgtgcgg tggatcgaag
     2461 agtggagcaa gcagttcctg gacgagaaga actacatgct gttcaccctg agaaacacct
     2521 acaacgagat gaatatcatc cacgagaaca actgcaagca gtacaataag tgggtgcaga
     2581 accggaagaa agagtggacc ttcctgagca acgagtttaa caagatcttc cccgagcgga
     2641 acgtgcagat ccacatcagc aacatcttca aagagtataa agaaaacaac gtggacatca
     2701 tctttggcac cctgaactac gagtacaata acttctgcaa agagaagccc gaactcgtca
     2761 gcgccgccaa gtacaacctg aaggccccca acgccaagag cccccggatc tacaagagca
     2821 aagagcacga ggaatccagc gtgttcggct gcaagaccaa gatcagcaaa gtgaagaaga
     2881 agtggaactg ctacagcaat aacaaagtga ccaagcccga gggcgtgtgc ggacctcctc
     2941 ggagacagca gctgtgcctg ggctacatct tcctgatccg ggacggcaac gaggaaggcc
     3001 tgaaggacca catcaataag gccgccaact acgaggccat gcacctgaaa gagaagtacg
     3061 aaaacgctgg cggcgacaag atctgcaacg ccatcctggg cagctacgcc gacatcggcg
     3121 atatcgtgcg gggcctggac gtgtggcggg acatcaacac caacaagctg tccgagaagt
     3181 tccagaaaat cttcatgggc ggagggaaca gccggaagaa gcagaacgac aataacgagc
     3241 ggaacaagtg gtgggaaaag cagcggaacc tgatctggtc cagcatggtc aagcacatcc
     3301 ccaagggcaa gacttgcaag cggcacaaca atttcgagaa gatcccccag ttcctgcggt
     3361 ggctgaaaga atggggcgac gagttctgcg aggaaatggg caccgaggtc aagcagctgg
     3421 aaaagatctg cgagaacaag aactgcgccg agaagaagtg caaaaacgcc tgcagcagct
     3481 acgagaagtg gatcaaagag cggaagaacg aatacaatct gcagagcaag aagttcgaca
     3541 gcgacaagaa gctgaacaag aagaacaacc tgtacaacaa gtttgaggac agcaaggcct
     3601 atctgcggag cgagtccaag cagtgcagca acatcgagtt caacgacgag acattcacct
     3661 tccccaacaa gtataaagag gcctgcatgg tctgcgagaa tcccagcagc agcaaggccc
     3721 tgaagcccat caagaccaac gtgttcccca tcgaggaaag caagaagtcc gagctgtcca
     3781 gcctgaccga caagagcaag aacaccccca acagcgcagg cggcggaaac tacggcgatc
     3841 ggcagatcag caagcgggac gacgtgcacc acgacggccc caaagaagtg aagtctggcg
     3901 agaaagaggt gcccaagatc gacgccgccg tgaaaaccga gaatgagttc accagcaacc
     3961 ggaacgacat cgagggcaaa gagaaaagca agggcgacca cagcagccct gtgcacagca
     4021 aggacatcaa gaacgaggaa ccccagcggg tggtgtccga gaatctgcct aagatcgagg
     4081 aaaagatgga aagcagcgac agcatcccca tcacccacat cgaggccgag aagggccaga
     4141 gcagcaacag cgccgacaac gaccctgctg tggtgtctgg ccgggagagc aaggacgtga
     4201 atctgcacac cagcgagaga attaaagaga atgaggaagg cgtcatcaag accgacgagt
     4261 caagcaagtc tatcgagatc tccaagatcc ccagcgatca gaacaaccac gccgacctga
     4321 gccagaacgc caacgaggac tccaaccagg gcaacaaaga gacaatcaac ccccccagca
     4381 ccgagaagaa tctgaaagag atccactaca agaccagcga ctccgacgac cacggcagca
     4441 agatcaagag cgagatcgag cccaaagagc tgaccgagga aagccccctg accgataaaa
     4501 agaccgagag cgccgccatc ggggacaaga accacgagag cgtgaagtcc gccgacatct
     4561 tccagtccga gatccacaac agcgacaacc gggaccggat cgtgtctgag agcgtggtgc
     4621 aggactctag cggctccagc atgagcaccg agtccatccg gactgataac aaggacttca
     4681 agacctccga ggatatcgcc cccagcatca acggccacga gaagatcggc agcagtgccg
     4741 acgatagagg cagcgaggac aagagcatca tcgacaagga cagcgagaac ttcgagaaca
     4801 ataaggcctc ccacagcgac atcaagcaga gcgacaacga gggcagcacc gactacgagt
     4861 ccctgaccga agagagcccc aagggcgatc tggaaagcgt gtcccccagc agcatcgaca
     4921 tggacctgaa gcctaacaag gccagccccg tgaccagctt cgatcatgag aaaatctccg
     4981 tgagcccccc caatgtggcc gtgacctacg acgagggcga caagagacag ggcatcagcg
     5041 acgactctag cattcaccac gagatcgacc ccgagagaaa tctgcactac gagagcttca
     5101 tcagcgaggg cggcctggaa gagggcgaca tcgagaaaga gaaggggaaa gaggacggca
     5161 gcctgatccc catcagcccc gagaagatca acgacttcgg caagcgggag aacatcgtgg
     5221 acctgagcgt gtccgagcgg gtggacgaca acagagccga catctccaac gtggtgtcag
     5281 aggaacagga aagcagcatc atcagcagcc ggaatggcgc cgagggcatc aacaatgccg
     5341 aggaactgaa gtccgaggaa cacaccagcg tggacgtgaa caacagggac gagcacaaca
     5401 agcaggaaaa cctcgtcagc agctccaccc agcaggaaag cgagcgggag aagagagaga
     5461 aaaaagagaa cgccgacagc agccacgagt ccgaactgag cagcatcagc gaagtgggcg
     5521 agacaatccg gcggaacgat gccgaggcct ccgagaacga caagggcgag gacatcctgc
     5581 agtgcgagga acagcagatc gtgaccgaga aaaccaagct gatcgagcct accgtgaata
     5641 tcctgcagcc cagcacccct ctgggcgaga gccacaaaga gaagtctgag gaaatcgacg
     5701 acaaggataa cacaggcggc gaagtgaccc acaccgacgc caacaccttc cagtacagat
     5761 cctccgagtc cgtggaagtg atcaccaata agcccgacga gatggaaatg acaaccaagc
     5821 ccccctccca gtacattgag aaagaaatcg gcgagatcga cagcaccaag aatcaggaca
     5881 acgacgagca gagcaactcc atcatccccc tgatcaacaa gaacaatgaa gaggatggcg
     5941 tgagcatccc ctccacccgg aacgtgatgg aaagcggcag cttcgtgagc agaaacgagc
     6001 agatcatcga agagaaggac gacaagcaca ttaccgacga caccacaatc aaccccagcg
     6061 agaatggcct gaagggctac ggcgagatgc ccaacgacgc catcaagagc gtgaccatca
     6121 ccgagagccc cctgagagat gtggagcaga tgatcgagcc aatcgacggc aagggcaacg
     6181 agaagaataa tatcatcggc gagccccagg aaagcaccac cgagatccgg aagcagatgg
     6241 acggccccat cagcaacgtg aacatccctg aggaactgca ccctgtggcc gagggctcca
     6301 aactggaaga ggccaaagaa cggtccatgg acgacgccga caagggcacc atcacagagg
     6361 acatcaccgt ggtggaggac cccaatggca tcggcgaaca ccagaacctg aaagaagtgc
     6421 acgagcaggc cagcgaactg aatacctaca acagcctgga cggccggacc aatgtggaag
     6481 tgaaagagcg gctggacgaa aaccccggct ccatccccaa cgaccggatc accaccgagc
     6541 acatcgagct ggataaagag aaagagattc acgagcctaa cgagctggac gcccacaacg
     6601 gcgagcagga agagatgatc cggaacgagg tgtccgacaa ccggatggat gagcagatca
     6661 gccgggacaa cgaggccaga cagctgaacc aggatcacga gagcgacagc aaggatgaga
     6721 tcatcgataa gcgggagatg gaaaatctgg aagagaaccc caactccgcc agcgacagcc
     6781 tggaaaatcc cgagggaaaa gaaaagggca agatcgagca cacccacagc agcgaagaac
     6841 tggacagcgt gcgggacgag acatacaagt acaagggcat cgaaaaccag atcaccgaga
     6901 cagagatcga gagcgtggaa cagcaggaca ccaacatccc cggcaacagc aaagaaaccg
     6961 aggtggacaa ctccagggcc gacatggaag aggaaaagga cgtcaagatc aagcagatca
     7021 ttaccgagga atccgaggaa gaactcgaga tctctaagga caccaccacc tcccactccg
     7081 agaagcccaa catcgaggaa cagagcgtga atatcgtgga ctccaagaac gagatcaatg
     7141 tgcagatcga gaaaaacgtg cagaatgagc agaacgaggg cgatcccatc atcctgctgg
     7201 aagaacagaa caagaatatc gccattctgg aaaaccagaa aaatgagtac aacaacccca
     7261 gccagctgtc ccacaaagag cgcacactgc tggaagtgga cgacctggaa ggcagcatgg
     7321 acaccgactg cctgacctcc gaactgaaca aaaataagtg cgactccatc cagatcatcc
     7381 ccgaggccag caacaccgat aacaagctga ataaggatat caccgagaac aaggatgact
     7441 tctccgagat cgagaagtcc gtgggcgaga tccacgaaaa cggcaaggat ctgctgaaca
     7501 aagagagcgc cgagagcgac gatgtgcccg tgcagaacaa gatcgaacac gatagcgaga
     7561 acgctggcgt gatcgaccag tacgagtacc ggaccgatta cgaggaaaac gagaaagtca
     7621 acttcgtggg cctgcccggc aagcctgagg aagagagaag cgacgtcacc agaggcagcg
     7681 tgcctggcag cgtggatgcc ttcgagaaaa agctgcacga gcccctggac agcaacgaga
     7741 aaaacaaaga ggaactcaac gtcgccaaag agatcgagta ccccgtcggc ctggatatcc
     7801 tgggcatcga cagcgagagc agcgattccg tgaccatcta caaaaagccc accgacacct
     7861 tcgtggagaa cgtgcacgtg ctgtccagag acatcaagag cctgttcgag aacgaaaaga
     7921 ccgtgggcct gaacgagcag aagaaagaga cagagaagaa cattgccggc ggaatgatcg
     7981 acgtgctcaa caataagaac atcgacgacg accagagcgt gcggctgtcc acctccctgt
     8041 accacaaggg cctggatgac gaccagtccg gcagactgag caccgccctg tatgataagg
     8101 ggctggacga cgatcagcac tatctgcacg acaacagcta caataacaat atcaacaaca
     8161 ttaagaatga ggtgcagtgt aaagagaact gcggccggaa cggcaatgtg gccagcttcg
     8221 tggataccac caccctgcgg gacgtgaagc agaacaaaga acacaccgag gaaacaaaac
     8281 tgattgagaa caccaagatc gataacacag aggtggacga cggcgtggat atcaccagcg
     8341 acaatcgggc cagcttcatc tccgtggaca actacgacga aaagaagaaa gaaaccatca
     8401 tcaatgagaa gaagaacgac cccaagccca gcagaaaaga agagaaagac gtcaaacacg
     8461 acgacagcga aaacgacaac aacaatctgg cctccaacat caagatcaaa gaacgcatcg
     8521 agaatatcta caactatatc aagaagctgt tcaacctggc cgacatcaac aattacgccg
     8581 agaaaaagga tatgccccgg tacaatctga acaacttcat caataccaag agctacaccc
     8641 tgttcgaaaa ctccaaagaa agcgagtacc acggcaatga gctgctgcag agcgagaaca
     8701 tccccgagca ccacaacaaa gccaataaca tcaaggtgtc cagcaacctg gacaataact
     8761 actaccgcta cgtgaacaag atcctggaag atatcatcaa tctggccaag aagaagaaaa
     8821 agtccatcaa cgatgccagc ttcgagtact gcgagagcat cctgagacac agcatcagct
     8881 ccagctccat ctacaaggat gagctgaagt acctgtgctt cagcatcctg tactactgcc
     8941 tgatctactt cgacaacacc gccgagtact acaactgcat caccaacgag ttcaataacg
     9001 ccctgtacat catgttccag accgacatga ccaatggcgc gccgtcgacc tccatcacgg
     9061 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     9121 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     9181 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     9241 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     9301 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     9361 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     9421 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     9481 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     9541 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     9601 gatcactgca tcatattctg gatgcacaga aaatggtgtg gaatcatcgt taagtctaga
     9661 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     9721 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     9781 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     9841 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     9901 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     9961 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
    10021 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
    10081 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
    10141 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
    10201 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
    10261 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
    10321 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
    10381 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
    10441 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
    10501 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
    10561 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
    10621 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
    10681 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
    10741 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
    10801 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
    10861 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
    10921 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
    10981 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
    11041 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
    11101 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
    11161 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
    11221 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
    11281 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
    11341 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
    11401 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
    11461 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
    11521 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    11581 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
    11641 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
    11701 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
    11761 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
    11821 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    11881 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
    11941 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
    12001 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    12061 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    12121 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    12181 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    12241 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    12301 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    12361 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    12421 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    12481 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    12541 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    12601 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    12661 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    12721 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    12781 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    12841 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    12901 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    12961 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    13021 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    13081 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    13141 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    13201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    13261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    13321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    13381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    13441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    13501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    13561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    13621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    13681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    13741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    13801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    13861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    13921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    13981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    14041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    14101 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    14161 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    14221 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
    14281 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg ga
//
