LOCUS       sequence-68768-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Alexeyev MF
  TITLE     The pKNOCK series of broad-host-range mobilizable suicide vectors 
            for gene knockout and targeted DNA insertion into the chromosome of 
            gram-negative bacteria.
  JOURNAL   Biotechniques. 1999 May;26(5):824-6, 828.
  PUBMED    10337469
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "1999-05"; volume: "26(5)"; pages: "824-6, 
            828"
COMMENT     Sequence Label: pKNOCK-Km
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..91
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     75..91
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(125..141)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(126..142)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(592..611)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt cccctccacc
       61 gcggtggcgg ccgctctaga actagtggat cccccgggct gcaggaattc gatatcaagc
      121 ttatcgatac cgtcgacctc gagggggggc ccggtaccga ggacgcgtcg aataattccg
      181 ctagcttcac gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg
      241 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
      301 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
      361 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
      421 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
      481 atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg
      541 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
      601 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
      661 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactccaa
      721 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
      781 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
      841 ctcctgtcat ctcaccttgc t
//
