LOCUS       sequence-68770-e        7479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length PTRAMP 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: PTRAMP-bio
FEATURES             Location/Qualifiers
     source          1..7479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2859..2914
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2913..2932)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5150..5168)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5236..5340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5341..5409,5410..6201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5559..5578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6372..6960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6861..6880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7114..7131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7248..7269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7284..7289,7290..7307,7308..7314)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7322..7338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7327..7349
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7346..7362
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7346..7362
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaatg acctgtgcag ctgcaacctg aaagtgcgga
     1381 acaactggga gcacttcctc caggtgtacc agaacaacgg caacttcaag atggaaatcc
     1441 tggaacccga gtgcaccatc cagaagaatc tgcccagcga ggaagagaaa gaggaattca
     1501 tcgacttcga ccggaaagag ctgctgctgc acgacttcgt ggtcatcaag aacagccagt
     1561 tcaccgacga gaaaaccctg gaactgtacc tgagcaaaga ggaaaacaac aaggcctacc
     1621 tgatgctgac cttctacctg ggcgacctga agctgatgat cggccacaac agcccctacg
     1681 agatcagcct gatcctgaac gtggccaccg tgaacaagaa catggccaac tgcaagaaca
     1741 acgcctacaa catcgtgctg ctgaaaacca ccgacgtgtt caacaccgcc gacctggaaa
     1801 ttctggaagg ccccatccag ttcagcctgg gcaagagcag cggcgccttc agaatcaacg
     1861 tggccaactt cttcctgaac aatgcctgga aggccttcac caaggacaag atcagcttcc
     1921 tgatcaagcc cgaggaaaac tgctacacca tcctggaaaa caagatcaac aagccccagc
     1981 tgctgatcga gaagaaaacc accttctaca gcgagtgggg cgagtggagc aactgcgcca
     2041 tggactgcga ccaccccgac aacgtgcaga tccgggagcg ggagtgcatc caccctagcg
     2101 gcgactgctt caagggcgat ctgaaagaga gccggccctg catcatccct ctgcccccct
     2161 gcaacgagct gttcagccac aaggacaatt ctggcgcgcc gtcgacctcc atcacggcct
     2221 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     2281 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     2341 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     2401 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     2461 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     2521 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     2581 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     2641 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     2701 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggat
     2761 cactgcatca tattctggat gcacagaaaa tggtgtggaa tcatcgttaa gtctagacac
     2821 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     2881 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     2941 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     3001 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     3061 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     3121 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     3181 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     3241 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     3301 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     3361 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     3421 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     3481 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     3541 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     3601 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     3661 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     3721 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     3781 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     3841 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     3901 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     3961 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     4021 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     4081 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     4141 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     4201 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     4261 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     4321 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     4381 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     4441 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     4501 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     4561 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4621 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4681 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4741 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     4801 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     4861 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     4921 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     4981 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5041 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     5101 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     5161 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5221 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5281 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5341 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5401 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5461 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5521 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5581 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5641 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5701 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5761 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5821 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5881 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5941 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6001 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6061 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6121 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6181 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6241 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6301 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6361 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6421 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6481 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6541 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6601 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6661 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6721 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6781 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6841 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6901 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6961 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7021 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7081 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7141 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7201 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7261 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7321 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     7381 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     7441 tgttgccatt gctgcaggcg cagaactggt aggtatgga
//
