LOCUS       sequence-68773-e        8562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated partial RH1 ectodomain with
            no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RH1-bio
FEATURES             Location/Qualifiers
     source          1..8562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3942..3997
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3996..4015)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6233..6251)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6319..6423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6424..6492,6493..7284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6642..6661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7455..8043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7944..7963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8197..8214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8331..8352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8367..8372,8373..8390,8391..8397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8405..8421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8410..8432
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8429..8445
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8429..8445
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcccagg aaagctacag cagcaacgag aagatccgga
     1381 aggactacag cgacgacaac aactacgagc ccacccccag ctacgagaag cggaagaaag
     1441 agtacggcaa ggacgagagc tacatcaaga actaccgggg caacaacttc gcctacgacc
     1501 tgagcaagaa cagcgccatc ttcctgcaca tgggcaacgg cgccaacagc aagaccctga
     1561 agcggtgcaa caagaagaag aacatcaaga ccaactttct gcggcccatc gaggaagaga
     1621 aaaccgtcct gaacaactac gtgtacaagg gcgtgaactt cctggacacc atcaagcgga
     1681 acgacgccag ctacaagttc gatgtgtaca aggacaccag ctttctgaag aaccgggagt
     1741 acaaagaact gatcaccatg cagtacgact acgcctacct ggaagccacc aaagaggtgc
     1801 tgtacctgat ccccaaggac aaggactacc acaagttcta caagaacgag ctggaaaaga
     1861 tcctgttcaa cctgaaggac agcctgaagc tgctgcggga gggctacatc cagagcaagc
     1921 tggaaatgat cagaatccac agcgacatcg acatcctgaa cgagttccac cagggcaaca
     1981 tcatcaacga caactacttc aacaacgaga tcaagaaaaa gaaagaagat atggaaaagt
     2041 acatccgcga gtacaacctg tacatctaca agtacgagaa ccagctgaag atcaagatcc
     2101 agaaactgac caacgaggtg tccatcaacc tgaacaaggc cacatgcgag aagaactgct
     2161 acaactacat cctgaagctc gagaagtaca agaatatcat caaggacaag atcaacaagt
     2221 ggaaggatct gcccgagatc tacatcgacg ataagagctt cagctacacc ttcctgaagg
     2281 acgtgatcaa caacaagatc gatatctaca agaccatcag cagcttcatc agcacccaga
     2341 agcagctgta ctacttcgag tacatctata tcatgaacaa gaacaccctg aatctgctgt
     2401 cctacaacat ccagaaaacc gacatcaaca gcgccagcaa gtacacctac accaagagcc
     2461 actttctgaa ggacaaccac atcctgctgt ccaagtacta caccgccaag ttcatcgata
     2521 ttctcaacaa ggcctactac tacaatctgt acaagaacaa gatcctgctg ttcaacaagt
     2581 acatcatcaa gctgcggaac gacctgaaag agtatgcctt caagagcatc cagttcatcc
     2641 aggataagat taagaagcac aaggacgagc tgtccatcga gaacatcctg caggaagtga
     2701 acaacatcta catcaagtac gacaccagca tcaacgagat ctccaagtac aacaacctga
     2761 tcatcaacac tgatctccag atcgtgcagc agaagctgct ggaaatcaag cagaaaaaga
     2821 acgacatcac ccacaaggtg cagctgatca accacatcta caaaaacatc cacgacgaga
     2881 tcctgaataa gaagaacaac gaaatcacca agatcatcat caacaacatc aaggaccaca
     2941 agaaagatct gcaggatctg ctgctgttta tccagcagat caagcagtac aatatcctga
     3001 ccgaccacaa gatcacccag tgcaacaact actacaaaga aatcatcaaa atgaaagagg
     3061 acatcaatca catccatatc tacatccagc ccatcctgaa caatctgcac accctgaaac
     3121 aggtgcagaa caataagatc aagtatgagg aacacatcaa acagatcctc cagaagatct
     3181 acgacaagaa agagagcctg aagaagatca tcctgctgaa ggacgaggcc cagctggata
     3241 tcactctgct ggatgacctc atccagaagc agaccggcgc gccgtcgacc tccatcacgg
     3301 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     3361 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     3421 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     3481 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     3541 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     3601 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     3661 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     3721 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     3781 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     3841 gatcactgca tcatattctg gatgcacaga aaatggtgtg gaatcatcgt taagtctaga
     3901 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     3961 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     4021 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     4081 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     4141 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     4201 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     4261 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     4321 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     4381 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     4441 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     4501 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     4561 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     4621 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     4681 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     4741 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     4801 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     4861 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     4921 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     4981 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     5041 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     5101 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     5161 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     5221 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     5281 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     5341 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     5401 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     5461 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     5521 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     5581 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     5641 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     5701 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     5761 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     5821 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     5881 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     5941 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     6001 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     6061 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     6121 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     6181 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     6241 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6361 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6421 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6481 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6541 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6601 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6661 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6721 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6781 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6841 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6901 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6961 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7021 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7081 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7141 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7201 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7261 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7321 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7561 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8041 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8101 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8161 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8221 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8281 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8341 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8401 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8461 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     8521 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg ga
//
