LOCUS       sequence-68783-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  TITLE     Ralf Kuehn TALEN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-TALEN-Rep
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(158..174)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(562..595)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      601..610
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(627..643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
       61 ggaccgatcc agcctccgga ctctagagga tccggtactc gaggacactg cagagaccta
      121 cttcactaac aaccggtatg gtcgccagta gcttggcact ggccgtcgtt ttacaacgtc
      181 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      241 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      301 tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc
      361 tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg
      421 gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg
      481 ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc
      541 tataaaaccg gtacagttcg aataacttcg tatagcatac attatacgaa gttataccgg
      601 gccaccatgg tcgcgagtag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
      661 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      721 aata
//
