LOCUS       sequence-68785-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     Ralf Kuehn TALEN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-RabZFN-A
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             74..94
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgccttctt ctttttccta cagatcctta attaataata cgactcacta taggggccgc
       61 caccatgaga tctcctaaga aaaagaggaa ggtggcggcc gctgactaca aggatgacga
      121 cgataaacca tggggtggcg gaggtagtcc atttcagtgt cgaatctgta tgagaaactt
      181 ttctgacaga tccaatcttt catctcatat ccgtacacac accggggaaa agccatttgc
      241 atgtgacatc tgtggccgaa agtttgctcg tagccactct ctcttgcggc atactaaaat
      301 ccacaccggg gagaagccat tccagtgccg catttgtatg cggaatttct ccaggagtga
      361 ctcccttagc gcacacatcc ggacccatac aggagagaag cccttcgcat gtgacatctg
      421 cggcaggaag ttcgcaactt cagggtctct gactcggcac accaaaatac ataccgggcg
      481 ccagaagcca ttccaatgta gaatctgtat gcggaatttc agtcaatccg gcaatcttgc
      541 ccgccacatt cgcacacaca caggagagaa gccttttgcg tgcgacattt gtgggcgcaa
      601 atttgcccag tcaggacatc tttcacgcca cactaaaatc cacctgaggg gatcccagct
      661 cgtcaagtca gaactggaag agaagaagtc agaactgcga cataaactca aatacgtccc
      721 ccatgaatac atcgaactga tcgagattgc taggaactcc acccaggaca gaatcctcga
      781 gatgaaagtg atggaattct ttatgaaagt ctacgggtat cgg
//
