LOCUS       sequence-68792-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Ralf Kuehn TALEN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-Luc
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(150..174)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(171..374)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(200..220)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        375..754
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag ctaattcggg gccgcggagg ctggatcggt cccggtgtct tctatggagg
      121 tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt
      181 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
      241 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      301 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      361 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      421 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      481 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
      541 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
      601 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      661 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
      721 gattactatt aataactagt caataatcaa tgtcaacatg gcggtaatgt tggacatgag
      781 ccaatataaa tgtacatatt atgatatgga tacaacgtat gcaatgggcc aagctcctcg
      841 agcccgggct agcacgcgta agagctcggt acctatcgat agagaaatgt tctggcacct
      901 gcacttgcac tggggacagc ct
//
