LOCUS       sequence-68797-e        9645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated partial RH2b ectodomain 
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RH2b-bio
FEATURES             Location/Qualifiers
     source          1..9645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5025..5080
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5079..5098)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7316..7334)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7402..7506
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7507..7575,7576..8367)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7725..7744)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8538..9126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9027..9046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9280..9297
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9414..9435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9450..9455,9456..9473,9474..9480)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9488..9504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9493..9515
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9512..9528
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9512..9528
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcccatt ttgtgggcca gagcagcaat acacacggcg
     1381 ccagcagcgt gaccgacttc aacttcgccg aggaaaagaa cctgaagtcc ttcgagggca
     1441 agaacaacaa caacgacaac tacgccagca tcaaccggct gtaccggaag aaaccctaca
     1501 tgaagcggag cctgatcaac ctggaaaacg acctgttccg gctggaaccc atcagctaca
     1561 tccagcggta ctacaagaag aacatcaacc gggccgacat cttccacaac aagaaagagc
     1621 ggggcagcaa ggtctacagc aacgtggcca gcttccacag cttcatccag gaaggcaaag
     1681 aggaagtcga agtctttagc atctggggca gcaacagcgt gctggaccac atcgacgtgc
     1741 tccgcgacaa tggagctgtg gtgtttagcg tgcagcccta ctacctggac atctatacat
     1801 gcaaagaggc catcctgttc accacaagct tctacaagga cctggacaag agcagcatca
     1861 ccaagatcaa cgaggacatc gagaagttca acgaggaaat catcaagaac gaggaacagt
     1921 gcctcgtggg cggcaagacc gacttcgaca atctgctgat cgtgctggaa aacgccgaga
     1981 aggccaacgt gcgcaagacc ctgttcgaca acaccttcaa cgactacaag aacaagaagt
     2041 cctccttcta caactgcctg aagaataaga agaacgacta cgacaagaag atcaagaaca
     2101 ttaagaacga gatcaccaag ctgctgaaga acatcgagag caccggcaac atgtgcaaga
     2161 ccgagagcta cgtgatgaac aacaacctgt atctgctgag agtgaacgaa gtgaagtcca
     2221 cccccatcga tctgtacctg aaccgggcca aagagctgct ggaaagcagc agcaagctgg
     2281 tcaaccccat caagatgaag ctgggcgaca acaagaacat gtacagcatc ggctatatcc
     2341 acgacgagat caaggacatc atcaagcggt acaacttcca cctgaagcac atcgaaaagg
     2401 gcaaagagta catcaagcgg atcacccagg ccaacaatat cgccgacaag atgaagaagg
     2461 acgagctgat caagaagatc ttcgagagca gcaagcactt cgccagcttc aagtacagca
     2521 acgagatgat ctccaagctg gacagcctgt tcatcaagaa tgaagagatc ctgaacaacc
     2581 tgttcaacaa tatcttcaac atcttcaaga agaagtacga gacatacgtc gacatgaaaa
     2641 ccatcgagtc caagtacacc accgtgatga ccctgagcga gcatctgctg gaatacgcca
     2701 tggacgtgct gaaggccaac ccccagaagc ccatcgaccc caaggccaac ctggacagcg
     2761 aggtggtcaa gctgcagatc aagatcaatg agaagtccaa tgaactcgac aatgctatca
     2821 gccaggtcaa gaccctgatc atcatcatga agtcctttta cgacatcatc atcagcgaga
     2881 aggcctccat ggacgagatg gaaaagaaag agctgtccct gaacaactac atcgagaaaa
     2941 ccgactacat cctgcagacc tacaacatct ttaagagcaa gagcaacatc atcaacaaca
     3001 atgccaagaa cattgccagc aagtacatca ccatcgaggg cctgaagaac gatatcgatg
     3061 aactcaactc tctgattagc tacttcaagg acagccagga aacactgatc aaggacgacg
     3121 agctgaagaa aaatatgaaa accgattacc tgaacaacgt gaagtacatc gaagagaacg
     3181 tggcccacat caacgagatc atcctgctga aggacagcat cacccagcgg atcgccgata
     3241 tcgacgaact gaactccctg aacctgatca acatcaacga ctttatcaac gagaagaata
     3301 tcgcccagga aaaagtctcc tacaacctga acaagctgta caagggcagc ttcgaggaac
     3361 tggaaagcga gctgtcccac ttcctggaca ccaagtacct gttccacgag aagaaaagcg
     3421 tgaacgagct gcagactatc ctgaataccg ctaacaacga gtgcgccaag ctgaacttca
     3481 tgaagtctga caacaacaat aacaataata acgccaatat catcaatctg ctcaagaccg
     3541 agctgtctca tctgctgtct ctgaaagaga acatcattaa gaagctgctc aaccacatcg
     3601 agcagaacat ccagaatagc gccaataagt acaccatcac ctacaccgac atcaacaacc
     3661 ggatggaaga ttacaaagaa gagatcgaga gcctggaagt gtacaagcac accatcggca
     3721 acattcagaa agagtatatc ctgcacctgt acgagaacga caagaacgcc ctggccgtgc
     3781 acaataccgc catgcagatc ctgcagtaca aggacgccat ccagaacatc aaaaacaaga
     3841 tctccgacga catcaagatc ctgaagaaat acaaagagat gaaccaggat ctgctgaact
     3901 actacgagat cctggataag aagctgaagg ataacaccta catcaaagag atgcacaccg
     3961 ccagcctggt gcagatcacc cagtacatcc cttacgagga caagaccatc agcgagctgg
     4021 aacaggaatt caataacaac aaccagaagc tggacaatat cctgcaggac atcaacgcca
     4081 tgaacctgaa catcaacatt ctgcagaccc tgaacatcgg catcaacgcc tgcaacacca
     4141 acaacaagaa tgtggagcat ctgctgaaca agaaaatcga gctgaaaaac atcctgaacg
     4201 accagatgaa gatcatcaaa aacgacgata tcatccagga caacgagaaa gagaacttcg
     4261 ccaacgtcct gaaaaaagag gaagagaagc tggaaaaaga gctggacgat atcaagttca
     4321 acaacctgaa gatggatatc cacaaactgc tgaacagcgg cgcgccgtcg acctccatca
     4381 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     4441 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     4501 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     4561 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     4621 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     4681 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     4741 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     4801 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     4861 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     4921 ccggatcact gcatcatatt ctggatgcac agaaaatggt gtggaatcat cgttaagtct
     4981 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     5041 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     5101 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     5161 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     5221 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     5281 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     5341 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     5401 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     5461 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     5521 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     5581 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     5641 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     5701 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     5761 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     5821 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     5881 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     5941 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     6001 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     6061 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     6121 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     6181 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     6241 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     6301 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     6361 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     6421 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     6481 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     6541 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     6601 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     6661 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     6721 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     6781 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     6841 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6901 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     6961 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     7021 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     7081 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     7141 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     7201 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     7261 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     7321 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7381 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7501 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7561 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7621 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7681 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7741 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7801 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7861 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7921 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7981 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8041 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8101 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8161 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8221 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8281 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8341 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8401 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8461 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9181 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9241 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9301 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9361 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9421 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9481 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     9541 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     9601 caacgttgtt gccattgctg caggcgcaga actggtaggt atgga
//
