LOCUS       sequence-68798-e        9999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated partial RH4 ectodomain with
            no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RH4-bio
FEATURES             Location/Qualifiers
     source          1..9999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5379..5434
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5433..5452)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7670..7688)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7756..7860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7861..7929,7930..8721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8079..8098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8892..9480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9381..9400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9634..9651
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9768..9789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9804..9809,9810..9827,9828..9834)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9842..9858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9847..9869
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9866..9882
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9866..9882
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccatcc ccagcaaaga gaagaagaac gaccccgagg
     1381 ccgacagcaa gaacagccag aaccagcacg acatcaacaa ggcccaccac accaacaaca
     1441 actacgacct gaacatcaag gacaaggacg agaagaagcg gaagaacgac aacctgatca
     1501 acaattacga ctacagcctg ctgaagctgt cctacaacaa gaaccaggac atctacaaga
     1561 atatccagaa cggccagaag ctgaaaaccg acatcatcct gaacagcttc gtgcagatca
     1621 acagcgccaa catcctgatg gacgagatcg agaactacgt gaagaagtac accgagagca
     1681 accggatcat gtatctgcag ttcaagtaca tctatctgca gagcctgaat atcgccgtga
     1741 gcttcgtgcc ccccaacagc cccttccggt cctactacga caagaacctg aacaaggaca
     1801 tcaatgaggc ctgccacagc atccagaccc tgctgaacaa tctgatcagc agcaagatca
     1861 tcttcaagat gctggaaacc accaaagagc agatcctgct gctgtggaac aacaagaaga
     1921 tcagccagca gaactacaac caggaaaatc aggaaaagtc caagatgatc gacagcgaga
     1981 acgagaagct ggaaaagtac accaacaagt tcgagcacaa catcaagccc cacatcgagg
     2041 acatcgagaa gaaagtgaac gagtacatca acaacgccga ctgccacctg acctgtagca
     2101 agtacaagac catcatcaac aactacatcg acgagatcat caccaccaac accaatatct
     2161 acgagaacaa gtacaatctg ccccaggaac ggatcatcaa gaattacaac cacaacggca
     2221 tcaataacga cgacaacttc atcgagtaca atatcctgaa cgccgacccc gacctgagaa
     2281 gccacttcat caccctgctg gtgtcccgga agcagctgat ctacatcgag tatatctact
     2341 tcatcaataa gcacatcgtg aacaagatcc aggaaaactt caagctgaac cagaataagt
     2401 acatccactt tatcaacagc aacaatgccg tgaacgccgc caaagagtac gagtacatta
     2461 tcaagtacta caccaccttc aagtatctgc agacactgaa caaggccctg tacgacagca
     2521 tctacaagca caagatcaat aactacgccc acaacatcga ggatctgatc aaccagctgc
     2581 agcacaagat taacaacctg atgattatca gcttcgacaa gaacaaggcc agcgacctga
     2641 tgctgcagtg caccaacatc aagaagtata ccgacgacat ctgcctgagc atcaagccca
     2701 aggccctgga agtggagtac ctgagaaaca tcaacaaaca catcaataag aacgagttcc
     2761 tcaacaagtt catgcagaac gaggccttca agaagaacat cgacgacaag atcaaagaga
     2821 tgaacaacat ctacgataac atctacatca tcctcaagca gaagtttctg aacaagctga
     2881 atgagatcat ccagaaccac aagaataagc aggaaaccaa gctgaacacc gccaccatcc
     2941 aggaactgct gcagctgctg aaggacatta aagagatcca gaccaagcag atcgacacca
     3001 agatcaacac cttcaacatg tactacaacg acatccagca gatcaagatc aagattaacc
     3061 agaacgagaa agaaatcaag aaggtgctgc cccagctgta catccccaag aacgagcagg
     3121 aatacatcca gatctacaaa aacgagctga aggaccggat caaagaaacc cagacaaaga
     3181 tcaacctgtt caagcagatt ctggaactga aagagaaaga gcactacatc accaacaagc
     3241 acacctacct gaacttcgcc cacaagacca ttcagcagat cctccagcag cagtacaaga
     3301 acaacgccca ggaaaagaac accctggccc agtttctgta caacgccgat atcaagaaat
     3361 acatcgatga gctgatcccc atcacccagc agattcagac caagatgtac accaccaata
     3421 acatcgagca catcaaacag atcctgatca attacatcca ggaatgcaag cccatccaga
     3481 acattgccga gcacaccatc tacaccctgt accaggaaat caagaccaac ctggaaaata
     3541 tcgagcagaa aattatgcag aatattcagc agaccaccaa ccggctgaag atcaacatca
     3601 aaaagatctt cgaccagatc aaccagaagt acgacgacct gaccaagaat atcaaccaga
     3661 tgaacgatga gaagatcggc ctgcggcaga tggaaaacag actgaagggc aagtacgagg
     3721 aaattaagaa ggccaatctg caggaccggg acatcaagta cattgtgcag aacaacgacg
     3781 ccaataacaa caacaataac atcatcatta tcaacggcaa taaccaggcc ggcgactaca
     3841 atcacatcct gttcgactac acccacctgt gggacaatgc ccagttcacc cggaccaaag
     3901 agaacatcaa taacctgaag gacaacatcc agattaatat caacaatatc aagagcatca
     3961 tccggaatct gcagaatgag ctgaacaact acaacaccct gaagtccaac agcatccaca
     4021 tctacgacaa aatccacacc ctggaagaac tgaagatcct gacccaggaa attaacgata
     4081 agaacgtgat ccggaagatc tacgacatcg agacaatcta ccagaacgat ctgcacaata
     4141 tcgaggaaat catcaaaaat atcgccagca tctactataa gatcaatatc ctcaacatcc
     4201 tcatcatctg catcaagcag acctacaaca ataataaggc catcgagagc ctgaagctca
     4261 aaatcaacaa cctggccaac tccgcccagg aatatatcaa tcagatcaag gccatcccca
     4321 ccaatctgct gcccgagcac attaagcaga aaagcgtgtc cgagctgaat atctacatga
     4381 agcagatcta tgacaagctg aacgagcacg tgatcaataa tctgtacacc aagtccaagg
     4441 acagcctgca gttctacatc aacgagaaga actacaataa caaccacgac gaccacaacg
     4501 acgatcacaa tgacgtgtac aacgatatca aagagaacga gatctataag aacaacaagc
     4561 tgtacgagtg catccagatc aagaaagacg tggacgagct gtacaacatc tatgatcagc
     4621 tgttcaagaa tatcgcccag aattacaata accacgccct gtccttcgtg cacagcatta
     4681 acaaccacat gctgagtatc ttccaggaca ccggcgcgcc gtcgacctcc atcacggcct
     4741 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     4801 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     4861 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     4921 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     4981 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     5041 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     5101 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     5161 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     5221 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggat
     5281 cactgcatca tattctggat gcacagaaaa tggtgtggaa tcatcgttaa gtctagacac
     5341 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     5401 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     5461 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     5521 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     5581 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     5641 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     5701 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     5761 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     5821 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     5881 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     5941 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     6001 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     6061 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     6121 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     6181 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     6241 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     6301 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     6361 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     6421 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     6481 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     6541 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     6601 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     6661 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     6721 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     6781 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     6841 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     6901 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     6961 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     7021 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     7081 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     7141 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     7201 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     7261 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     7321 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     7381 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     7441 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     7501 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     7561 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     7621 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     7681 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7741 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8041 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8101 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8161 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8401 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8461 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8701 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8761 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9001 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9541 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9601 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9661 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     9721 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9781 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9841 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     9901 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     9961 tgttgccatt gctgcaggcg cagaactggt aggtatgga
//
