LOCUS       sequence-68799-e        8139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RH5 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RH5-bio
FEATURES             Location/Qualifiers
     source          1..8139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3519..3574
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3573..3592)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5810..5828)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5896..6000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6001..6069,6070..6861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6219..6238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7032..7620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7521..7540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7774..7791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7908..7929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7944..7949,7950..7967,7968..7974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7982..7998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7987..8009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8006..8022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8006..8022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccttcg agaacgccat caagaaaacc aagaaccagg
     1381 aaaacaacct ggccctgctg cccatcaaga gcaccgagga agagaaggac gacatcaaga
     1441 acggcaagga tatcaagaaa gagatcgaca acgacaaaga gaacatcaag accaacaacg
     1501 ccaaggacca cagcacctac atcaagagct acctgaacac caacgtgaac gacggcctga
     1561 agtacctgtt catccccagc cacaacagct tcatcaagaa atacagcgtg ttcaaccaga
     1621 tcaacgacgg catgctgctg aacgagaaga acgacgtgaa gaacaacgag gactacaaga
     1681 acgtggatta caagaatgtg aacttcctgc agtaccactt caaagagctg tccaactaca
     1741 atatcgccaa cagcatcgac atcctgcagg aaaaagaggg ccacctggac ttcgtgatca
     1801 tcccccacta cacctttctg gactactaca agcacctgag ctacaacagc atctaccaca
     1861 agagcagcac ctacggcaag tgtatcgccg tggacgcctt tatcaagaag atcaacgaag
     1921 cctacgacaa agtgaagtcc aagtgcaacg acatcaaaaa tgacctgatc gccaccatca
     1981 agaagctgga acacccctac gacatcaaca acaagaacga cgacagctac agatacgaca
     2041 tcagcgagga aatcgacgac aagagcgaag agacagacga cgagacagag gaagtggagg
     2101 acagcatcca ggacaccgac agcaaccacg cccccagcaa caagaaaaag aacgacctga
     2161 tgaaccgggc cttcaagaaa atgatggacg agtacaacac caagaagaaa aagctgatca
     2221 agtgcatcaa gaaccacgag aacgacttca acaagatctg catggacatg aagaactacg
     2281 gcaccaacct gttcgagcag ctgtcctgct acaacaacaa cttctgcaac accaacggca
     2341 tcagatacca ctacgacgag tacatccaca agctgatcct gagcgtgaag tctaagaacc
     2401 tgaacaagga cctgagcgac atgaccaata tcctgcagca gagcgagctg ctgctgacca
     2461 acctgaataa gaagatgggc agctacatct acatcgatac catcaagttc atccacaaag
     2521 agatgaagca catcttcaac cggatcgagt accacacaaa gatcatcaac gacaagacca
     2581 agatcatcca ggataagatc aagctgaaca tctggcggac cttccagaag gacgagctgc
     2641 tgaagcggat cctggacatg agcaacgagt acagcctgtt catcacctcc gaccacctga
     2701 gacagatgct gtacaacacc ttctacagca aagagaagca cctgaacaac atcttccacc
     2761 acctgatcta cgtgctgcag atgaagttca acgacgtgcc tatcaagatg gaatacttcc
     2821 agacctacaa gaagaacaag cccctgaccc agggcgcgcc gtcgacctcc atcacggcct
     2881 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     2941 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     3001 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     3061 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     3121 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     3181 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     3241 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     3301 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     3361 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggat
     3421 cactgcatca tattctggat gcacagaaaa tggtgtggaa tcatcgttaa gtctagacac
     3481 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     3541 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     3601 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     3661 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     3721 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     3781 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     3841 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     3901 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     3961 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     4021 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     4081 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     4141 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     4201 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     4261 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     4321 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     4381 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     4441 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     4501 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     4561 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     4621 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     4681 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     4741 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     4801 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     4861 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     4921 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     4981 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     5041 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     5101 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     5161 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     5221 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     5281 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     5341 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5401 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     5461 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     5521 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     5581 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5641 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5701 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     5761 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     5821 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5881 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5941 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6001 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6061 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6241 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6541 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     8041 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     8101 tgttgccatt gctgcaggcg cagaactggt aggtatgga
//
