LOCUS       sequence-68800-e        8913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RAP1 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RAP1-bio
FEATURES             Location/Qualifiers
     source          1..8913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4293..4348
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4347..4366)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6584..6602)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6670..6774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6775..6843,6844..7635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6993..7012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7806..8394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8295..8314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8548..8565
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8682..8703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8718..8723,8724..8741,8742..8748)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8756..8772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8761..8783
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8780..8796
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8780..8796
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccatca acgtgaacgg cgacaacaac tacggcaaga
     1381 ccatcatcaa caacgacttc aacttcgacg actacaacta ctggaccccc atcaacaaga
     1441 aagagtttct gaacagctac gaggacaagt tcagcagcga gagcttcctg gaaaacaagg
     1501 ccagcgtgga cgacggcaac atcaacctgg ccgacaccag caccagcaac aaggcctcca
     1561 agaagggcca cggcagatct agagtgcgga gcgcctctgc cgccgctatc ctggaagagg
     1621 acgacagcaa ggacgacatg gaattcaagg ccagccccag cgtggtcaag accagcaccc
     1681 ctagcggcac acagaccagc ggcctgaagt ccagctctcc tagcagcacc aagagcagca
     1741 gcccctccaa cgtgaagtct gccagccccc acggcgagag caatagcgcc gaggaaagca
     1801 ccaccaagtc cagcaagaga agcgctagcg tcgctggcat tgtgggagcc gacgaggaag
     1861 ccccccctgc ccccaagaat accctgaccc ccctggaaga actgtacccc accaatgtga
     1921 acctgttcaa ctacaagtac agcctgaaca acatggaaga gaacatcaac atcctgaaga
     1981 acgagggcga cctggtggcc cagaaagagg aattcgagta cgacgagaac atggaaaagg
     2041 ccaagcagga caagaagaag gccctggaaa agatcggcaa agagtctgac gaggaacctt
     2101 tcatgttcag cgagaacaag tttctggaaa accaggtcaa agaacggaac gtggccggct
     2161 ccttcagccg gttcttcagc aagctgaacc ccttcaagaa agacgaagtg atcgagaaaa
     2221 ccgaggtgtc caagaaaacc ttcagcggca tcggcttcaa tctggccgag aaagaagcca
     2281 aggtcctggg cgtgggcgtg acctaccagg aataccccga gacaatgctg tacaactgcc
     2341 ccaacaacgc caacctgttc gacaccatcg agagcctgca gggcagagtg atcgacatca
     2401 agaaacggga gagcatgatc agcaccacct tcgagcagca gaaagaatgc ctgaagaata
     2461 tgggcgtgct ggacctggaa ctgaacgacg cccagtgcaa gttcggcacc tgtatcggca
     2521 gcttcggcga gcaccacctg agactgtacg agttcgagaa cgatctgctg aagttccacc
     2581 ccaacatcga ctacctgacc ctggccgacg gctacaagct gcagaagaac gacatctacg
     2641 agctgtccca cgtgaacttt tgcctgctga accccaagac cctggaagag ttcctgaaga
     2701 agaaagaaat caaggacctg atgggcggcg acgacctgat caagtacaaa gagaacttcg
     2761 acaacttcat gagcatcagc atcacctgtc acatcgagtc cctgatctac gacgacatcg
     2821 aggccagcca ggatatcgcc gccgtgctga agatcgccaa gagcaagctg cacgtgatca
     2881 cctccggcct gagctacaag gcccggaagc tggtgtacaa gatctacagc gagatccaga
     2941 agaaccccga cgagctgtac gagaaactga cctggatcta tgataatatc tacatgatca
     3001 agcggtacta caccgcctac gccctggaag gcgtgtgcag ctacctggaa cacgacaaga
     3061 gccagatgta caccgagctg cacatctaca acaagatcgt ggacagcgtg cggtactact
     3121 ccagctgctt caagaacgtg atcgtgtaca acgccatcat ctccggcatc cacgagaaga
     3181 tcaagcactt cctgaagctg gtgccccggc acaactttct gctggactac cacttcaaca
     3241 gcatcttcga gaaagagatc aagcccgcca agaagtacag caccagccac atctacttcg
     3301 accccaccgt ggccagctac gcctactaca acctggaccg gcggaccatg gtcacaatca
     3361 tcaacgacta cttcgaggcc aagaaaaaag aactgacagt gatcgtgtcc cggatgaaaa
     3421 ccgacatgct gtccctgcag aacgaagaga gcaagatccc caacgacaag tccgccaact
     3481 ccaagctggc cacccggctg atgaagaagt tcaaggccga gatccgggac ttcttcaaag
     3541 agatgcggat ccagtacgcc aagctgatca acatccggta cagaagccac ctgaagaaaa
     3601 actacttcgc cttcaagcgg ctggacggcg cgccgtcgac ctccatcacg gcctataaga
     3661 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     3721 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     3781 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     3841 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     3901 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     3961 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     4021 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     4081 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     4141 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggatcactgc
     4201 atcatattct ggatgcacag aaaatggtgt ggaatcatcg ttaagtctag acacgtgatt
     4261 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     4321 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     4381 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     4441 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     4501 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     4561 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     4621 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     4681 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     4741 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     4801 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     4861 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     4921 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     4981 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     5041 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     5101 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     5161 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     5221 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     5281 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     5341 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     5401 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     5461 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     5521 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     5581 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     5641 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     5701 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     5761 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     5821 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     5881 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     5941 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     6001 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     6061 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     6121 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     6181 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     6241 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     6301 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     6361 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     6421 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     6481 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     6541 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     6601 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     7021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     7321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7921 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8701 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8761 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     8821 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     8881 cattgctgca ggcgcagaac tggtaggtat gga
//
