LOCUS       sequence-68801-e        7764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RAP2 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RAP2-bio
FEATURES             Location/Qualifiers
     source          1..7764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3144..3199
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3198..3217)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5435..5453)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5521..5625
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5626..5694,5695..6486)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5844..5863)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6657..7245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7146..7165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7399..7416
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7533..7554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7569..7574,7575..7592,7593..7599)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7607..7623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7612..7634
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7631..7647
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7631..7647
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgaca agtgcgagac agagttcagc aagctgtacc
     1381 ccgagagcaa cagcctgacc ggcctgatct acgcccacac cgccaacgtg cacaagctgt
     1441 ctatgtgggt gtacttcatc tacaaccact tcagcagcgc cgacgagctg atcaagtacc
     1501 tggaaaagac caacatcaac accctggaaa acagcgacca cacctgtttc gccagagccg
     1561 tgaccctgta cctgttctac tactacctga aggacatcaa gagcatgctg tccaccgacg
     1621 actaccagag cttcttcaag aacaagttca aggatatcaa ccccctgttc atcaacgact
     1681 tcatcctgat cctgaacgac aagaaattca tggaaaacct ggacctgtac atcatgaaag
     1741 agagcgagcg ggagcacctg gtcatcaaga agaacccctt tctgcgggtg ctgaacaagg
     1801 ccagcaccac cacccacgcc acctacaaga gcaaccccta cttcatcgtg ggcagccggg
     1861 tgcacacccc ctacaaggac tacctgggcg acttcaacaa gtacaccgag atcagcgtgc
     1921 tgaactacgt gcgggactac aacttcctga tctatgccgg ctctcgcgag aactactaca
     1981 acagcgacat tgccggccct gccagatccg tgaacaacgt gatcagcaag aacaaggctc
     2041 tgggcctgcg gaagagaagc agcagcctgg ccctcgtggg caccaacaac aacgacccca
     2101 tcttcgccta ctgcgagaag gacaacaagg ccgagtacta cggcaccccc gatgacctga
     2161 tcaccagctt tttcagcatc atcaagacca agatgctgaa cagccacaag acctttctga
     2221 gacagttcga ctacgccctg tttcacaaga cctacagcat ccccaacctg aagggcttcc
     2281 ggttcctgaa gcacctgttc cagaagaaga acctggtcaa cttcgtgggc atgtacgaga
     2341 accacgtgtc cacagagatc aactttctgg ccgaggactt cgtggagctg ttcgacgtga
     2401 ccatggactg ctacagccgg cagtacagca acagagccgc cgagaacttc aaggccatcc
     2461 gggaactgaa cgtcctgggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2521 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     2581 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     2641 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     2701 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     2761 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     2821 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     2881 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     2941 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     3001 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggatcactg catcatattc
     3061 tggatgcaca gaaaatggtg tggaatcatc gttaagtcta gacacgtgat taattaagga
     3121 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     3181 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     3241 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     3301 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3361 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3421 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3481 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3541 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3601 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3661 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     3721 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     3781 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     3841 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     3901 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     3961 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     4021 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     4081 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     4141 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     4201 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     4261 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     4321 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4381 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4441 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4501 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4561 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4621 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4681 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     4741 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     4801 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4861 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4921 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4981 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     5041 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     5101 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     5161 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     5221 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     5281 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5341 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5401 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5461 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5521 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5581 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5641 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5701 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5761 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5821 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     5881 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5941 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6001 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6061 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6121 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     6181 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6241 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6301 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6361 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6421 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6481 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6541 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6601 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6661 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6721 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6781 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6841 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6901 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6961 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7021 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7081 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7141 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7201 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7261 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7321 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7381 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7441 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7501 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7561 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7621 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7681 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     7741 aggcgcagaa ctggtaggta tgga
//
