LOCUS       sequence-68804-e        9255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3)
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB62
FEATURES             Location/Qualifiers
     source          1..9255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..57
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     49..66
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     50..66
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        127..726
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     CDS             750..815
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             822..842
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(867..1907,1959..2945,2997..4058,4110..5120)
                     /codon_start=1
                     /product="S. pyogenes Cas9, codon optimized for C. elegans 
                     with synthetic introns"
                     /label=Cas9
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(5127..5429,5483..5557,5609..5728,5780..5995)
                     /codon_start=1
                     /product="monomeric enhanced GFP, optimized with synthetic 
                     introns for expression in C. elegans"
                     /label=mEGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGMDELYK"
     CDS             6002..6076
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="MSRRRKANPTKLSENAKKLAKEVEN"
     terminator      6086..6409
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     promoter        complement(6517..6535)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6556..6572)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6556..6572)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6569..6591)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6580..6596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6604..6610,6611..6628,6629..6634))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6649..6670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6787..6804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6958..7546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7038..7057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7717..8508,8509..8577))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8340..8359
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8578..8682)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8708..9163)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8845..8866)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9057..9076
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
       61 gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
      121 gtcgacgcac ctttggtctt ttattgtcaa cttccattgg ttcttccatt gtttctgtta
      181 aattaatgaa tttttcataa aataaagaca ttatacaata taaaaatgaa gaatttattg
      241 aaaataaact gccagagaga aaaagtatgc aacactcccg ccgagagtgt ttgaaatggt
      301 gtacggtaca ttttcgtgct aggagttaga tgtgcaggca gcaacgagag ggggagagat
      361 ttttttgggc cttgtgaaat taacgtgagt tttctggtca tctgactaat catgttggtt
      421 ttttgttggt ttattttgtt tttatctttg tttttatcca gattaggaaa tttaaatttt
      481 atgaatttat aatgaggtca aacattcagt cccagcgttt ttcctgttct cactgtttag
      541 tcgaattttt attttaggct ttcaacaaat gttctaactg tcttatttgt gacctcactt
      601 tttatatttt tttaattttt aaaaatatta gaagtttcta ggataatttt ttcgactttt
      661 attctctcta ccgtccgcac tcttcttact tttaaattaa attgtttttt tttcagttgg
      721 gaaacacttt gctcatctag aaaaaaatgg actacaagga ccacgacgga gactacaagg
      781 accacgacat cgactacaag gacgacgacg acaagatggc cccaaagaag aagcgtaagg
      841 tcggaatcca cggagtccca gccgccgaca agaagtactc catcggactc gacatcggaa
      901 ccaactccgt cggatgggcc gtcatcaccg acgagtacaa ggtcccatcc aagaagttca
      961 aggtcctcgg aaacaccgac cgtcactcca tcaagaagaa cctcatcgga gccctcctct
     1021 tcgactccgg agagaccgcc gaggccaccc gtctcaagcg taccgcccgt cgtcgttaca
     1081 cccgtcgtaa gaaccgtatc tgctacctcc aagagatctt ctccaacgag atggccaagg
     1141 tcgacgactc cttcttccac cgtctcgagg agtccttcct cgtcgaggag gacaagaagc
     1201 acgagcgtca cccaatcttc ggaaacatcg tcgacgaggt cgcctaccac gagaagtacc
     1261 caaccatcta ccacctccgt aagaagctcg tcgactccac cgacaaggcc gacctccgtc
     1321 tcatctacct cgccctcgcc cacatgatca agttccgtgg acacttcctc atcgagggag
     1381 acctcaaccc agacaactcc gacgtcgaca agctcttcat ccaactcgtc caaacctaca
     1441 accaactctt cgaggagaac ccaatcaacg cctccggagt cgacgccaag gccatcctct
     1501 ccgcccgtct ctccaagtcc cgtcgtctcg agaacctcat cgcccaactc ccaggagaga
     1561 agaagaacgg actcttcgga aacctcatcg ccctctccct cggactcacc ccaaacttca
     1621 agtccaactt cgacctcgcc gaggacgcca agctccaact ctccaaggac acctacgacg
     1681 acgacctcga caacctcctc gcccaaatcg gagaccaata cgccgacctc ttcctcgccg
     1741 ccaagaacct ctccgacgcc atcctcctct ccgacatcct ccgtgtcaac accgagatca
     1801 ccaaggcccc actctccgcc tccatgatca agcgttacga cgagcaccac caagacctca
     1861 ccctcctcaa ggccctcgtc cgtcaacaac tcccagagaa gtacaaggta agtttaaaca
     1921 tatatatact aactaaccct gattatttaa attttcagga gatcttcttc gaccaatcca
     1981 agaacggata cgccggatac atcgacggag gagcctccca agaggagttc tacaagttca
     2041 tcaagccaat cctcgagaag atggacggaa ccgaggagct cctcgtcaag ctcaaccgtg
     2101 aggacctcct ccgtaagcaa cgtaccttcg acaacggatc catcccacac caaatccacc
     2161 tcggagagct ccacgccatc ctccgtcgtc aagaggactt ctacccattc ctcaaggaca
     2221 accgtgagaa gatcgagaag atcctcacct tccgtatccc atactacgtc ggaccactcg
     2281 cccgtggaaa ctcccgtttc gcctggatga cccgtaagtc cgaggagacc atcaccccat
     2341 ggaacttcga ggaggtcgtc gacaagggag cctccgccca atccttcatc gagcgtatga
     2401 ccaacttcga caagaacctc ccaaacgaga aggtcctccc aaagcactcc ctcctctacg
     2461 agtacttcac cgtctacaac gagctcacca aggtcaagta cgtcaccgag ggaatgcgta
     2521 agccagcctt cctctccgga gagcaaaaga aggccatcgt cgacctcctc ttcaagacca
     2581 accgtaaggt caccgtcaag caactcaagg aggactactt caagaagatc gagtgcttcg
     2641 actccgtcga gatctccgga gtcgaggacc gtttcaacgc ctccctcgga acctaccacg
     2701 acctcctcaa gatcatcaag gacaaggact tcctcgacaa cgaggagaac gaggacatcc
     2761 tcgaggacat cgtcctcacc ctcaccctct tcgaggaccg tgagatgatc gaggagcgtc
     2821 tcaagaccta cgcccacctc ttcgacgaca aggtcatgaa gcaactcaag cgtcgtcgtt
     2881 acaccggatg gggacgtctc tcccgtaagc tcatcaacgg aatccgtgac aagcaatccg
     2941 gaaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagacca
     3001 tcctcgactt cctcaagtcc gacggattcg ccaaccgtaa cttcatgcaa ctcatccacg
     3061 acgactccct caccttcaag gaggacatcc aaaaggccca agtctccgga caaggagact
     3121 ccctccacga gcacatcgcc aacctcgccg gatccccagc catcaagaag ggaatcctcc
     3181 aaaccgtcaa ggtcgtcgac gagctcgtca aggtcatggg acgtcacaag ccagagaaca
     3241 tcgtcatcga gatggcccgt gagaaccaaa ccacccaaaa gggacaaaag aactcccgtg
     3301 agcgtatgaa gcgtatcgag gagggaatca aggagctcgg atcccaaatc ctcaaggagc
     3361 acccagtcga gaacacccaa ctccaaaacg agaagctcta cctctactac ctccaaaacg
     3421 gacgtgacat gtacgtcgac caagagctcg acatcaaccg tctctccgac tacgacgtcg
     3481 accacatcgt cccacaatcc ttcctcaagg acgactccat cgacaacaag gtcctcaccc
     3541 gttccgacaa gaaccgtgga aagtccgaca acgtcccatc cgaggaggtc gtcaagaaga
     3601 tgaagaacta ctggcgtcaa ctcctcaacg ccaagctcat cacccaacgt aagttcgaca
     3661 acctcaccaa ggccgagcgt ggaggactct ccgagctcga caaggccgga ttcatcaagc
     3721 gtcaactcgt cgagacccgt caaatcacca agcacgtcgc ccaaatcctc gactcccgta
     3781 tgaacaccaa gtacgacgag aacgacaagc tcatccgtga ggtcaaggtc atcaccctca
     3841 agtccaagct cgtctccgac ttccgtaagg acttccaatt ctacaaggtc cgtgagatca
     3901 acaactacca ccacgcccac gacgcctacc tcaacgccgt cgtcggaacc gccctcatca
     3961 agaagtaccc aaagctcgag tccgagttcg tctacggaga ctacaaggtc tacgacgtcc
     4021 gtaagatgat cgccaagtcc gagcaagaga tcggaaaggt aagtttaaac atgattttac
     4081 taactaacta atctgattta aattttcagg ccaccgccaa gtacttcttc tactccaaca
     4141 tcatgaactt cttcaagacc gagatcaccc tcgccaacgg agagatccgt aagcgtccac
     4201 tcatcgagac caacggagag accggagaga tcgtctggga caagggacgt gacttcgcca
     4261 ccgtccgtaa ggtcctctcc atgccacaag tcaacatcgt caagaagacc gaggtccaaa
     4321 ccggaggatt ctccaaggag tccatcctcc caaagcgtaa ctccgacaag ctcatcgccc
     4381 gtaagaagga ctgggaccca aagaagtacg gaggattcga ctccccaacc gtcgcctact
     4441 ccgtcctcgt cgtcgccaag gtcgagaagg gaaagtccaa gaagctcaag tccgtcaagg
     4501 agctcctcgg aatcaccatc atggagcgtt cctccttcga gaagaaccca atcgacttcc
     4561 tcgaggccaa gggatacaag gaggtcaaga aggacctcat catcaagctc ccaaagtact
     4621 ccctcttcga gctcgagaac ggacgtaagc gtatgctcgc ctccgccgga gagctccaaa
     4681 agggaaacga gctcgccctc ccatccaagt acgtcaactt cctctacctc gcctcccact
     4741 acgagaagct caagggatcc ccagaggaca acgagcaaaa gcaactcttc gtcgagcaac
     4801 acaagcacta cctcgacgag atcatcgagc aaatctccga gttctccaag cgtgtcatcc
     4861 tcgccgacgc caacctcgac aaggtcctct ccgcctacaa caagcaccgt gacaagccaa
     4921 tccgtgagca agccgagaac atcatccacc tcttcaccct caccaacctc ggagccccag
     4981 ccgccttcaa gtacttcgac accaccatcg accgtaagcg ttacacctcc accaaggagg
     5041 tcctcgacgc caccctcatt caccaatcca tcaccggact ctacgagacc cgtatcgacc
     5101 tctcccaact cggaggagac ctgcagatgt ccaagggaga ggagctcttc accggagtcg
     5161 tcccaatcct cgtcgagctc gacggagacg tcaacggaca caagttctcc gtctcaggag
     5221 agggagaggg agacgccacc tacggaaagc tcaccctcaa gttcatctgc accaccggaa
     5281 agctcccagt cccatggcca accctcgtca ccaccttcac ttacggagtc caatgcttct
     5341 cccgttaccc agaccacatg aagcgtcacg acttcttcaa gtccgccatg ccagagggat
     5401 acgtccaaga gcgtaccatc ttcttcaagg taagtttaaa cattaattaa tactaactaa
     5461 ccctgattat ttaaattttc aggacgacgg aaactacaag acccgtgccg aggtcaagtt
     5521 cgagggagac accctcgtca accgtatcga gctcaaggta agtttaaaca gttcggtact
     5581 aactaaccat acatatttaa attttcaggg aatcgacttc aaggaggacg gaaacatcct
     5641 cggacacaag ctcgaataca actacaactc ccacaacgtc tacatcatgg ccgacaagca
     5701 aaagaacgga atcaaggtca acttcaaggt aagtttaaac atgattttac taactaacta
     5761 atctgattta aattttcaga tccgtcacaa catcgaggac ggatctgtcc aactcgccga
     5821 ccactaccaa caaaacaccc caatcggaga cggaccagtc ctcctcccag acaaccacta
     5881 cctctccacc caatccgccc tctccaagga cccaaacgag aagcgtgacc acatggtcct
     5941 caaggagttc gtcaccgctg ccggaatcac ccacggaatg gacgagctct acaagctgca
     6001 gatgtcccgt cgtcgtaagg ccaacccaac caagctctcc gagaacgcca agaagctcgc
     6061 caaggaggtc gagaactagg aattcatgca agatcctttc aagcattccc ttcttctcta
     6121 tcactcttct ttctttttgt caaaaaattc tctcgctaat ttatttgctt ttttaatgtt
     6181 attattttat gactttttat agtcactgaa aagtttgcat ctgagtgaag tgaatgctat
     6241 caaaatgtga ttctgtctga tgtactttca caatctctct tcaattccat tttgaagtgc
     6301 tttaaacccg aaaggttgag aaaaatgcga gcgctcaaat atttgtattg tgttcgttga
     6361 gtgacccaac aaaaagagga aactttattg tgccgccaag aaaaaagtct cattgcctaa
     6421 gagaaacata tgtggttgga aatcttccag atcgtggatc actagtatgt ggttggaaat
     6481 cttccagatc gtggatcaga gctccagctt ttgttccctt tagtgagggt taatttcgag
     6541 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6601 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6661 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6721 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6781 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6841 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6901 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6961 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7021 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7081 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7141 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7201 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7261 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7321 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7381 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7441 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7501 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7561 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7621 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7681 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7741 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7801 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7861 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7921 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7981 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8041 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8101 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8161 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8221 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8281 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8341 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8401 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8461 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8521 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8581 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8641 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8701 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     8761 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8821 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8881 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8941 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     9001 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     9061 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     9121 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     9181 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9241 aaagggggat gtgct
//
