LOCUS       sequence-68807-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expesses the human codon usage Cas9 nuclease (Mali et al. Science 
            339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter
            in the plant tissue .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S
  TITLE     Targeted mutagenesis in the model plant Nicotiana benthamiana using 
            Cas9 RNA-guided endonuclease.
  JOURNAL   Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655.
  PUBMED    23929340
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08-8- 8"; volume: "31"; 
            issue: "8"; pages: "691-3"
COMMENT     Sequence Label: pK7WGF2::hCas9
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..42
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctgtacaa ggatatcaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca
       61 ccatggacaa gaagtactcc attgggctcg atatcggcac aaacagcgtc ggctgggccg
      121 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
      181 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg
      241 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
      301 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
      361 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
      421 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
      481 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
      541 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
      601 atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc
      661 cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc
      721 ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta
      781 atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg
      841 aagatgccaa gcttca
//
