LOCUS       sequence-68808-e        8125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB67
FEATURES             Location/Qualifiers
     source          1..8125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        730..1034
                     /label=hsp16-48 promoter
                     /note="promoter for the C. elegans heat shock protein 16-48
                     gene (Russnak and Candido, 1985)"
     CDS             1048..1113
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1120..1140
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1165..2205,2257..3243,3295..4356,4408..5418)
                     /codon_start=1
                     /product="S. pyogenes Cas9, codon optimized for C. elegans 
                     with synthetic introns"
                     /label=Cas9
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5425..5499
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="MSRRRKANPTKLSENAKKLAKEVEN"
     terminator      5509..5832
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     promoter        complement(5940..5958)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5979..5995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5979..5995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5992..6014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6003..6019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6027..6033,6034..6051,6052..6057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6072..6093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6210..6227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6381..6969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6461..6480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7140..7931,7932..8000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7763..7782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8001..8105)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgc
      661 tggacggaaa tagtggtaaa gtgacatgat tgtagtttga agatttcaca attagagtga
      721 atgttgtttg gttcggtttt gtcactgtat ttatactcat ttccaccttt ttctagaaca
      781 ttcgagctgc ttcttacaaa aggaggcgac tcacattcag aacattgaga aatagtgtgc
      841 gactgaagaa acccagatac tttttcaatc tgcgtctctt tgcacctatg gggtgtattt
      901 tgaaatgaat gcatctagga ccttctagaa cattctaaac ggctgcagga tacgggtata
      961 taagccaatc gtgttcagag gaaaccaata cactttgttc aagtgcttac tgttcattct
     1021 ctaaacttca agaactagaa aaaaatggac tacaaggacc acgacggaga ctacaaggac
     1081 cacgacatcg actacaagga cgacgacgac aagatggccc caaagaagaa gcgtaaggtc
     1141 ggaatccacg gagtcccagc cgccgacaag aagtactcca tcggactcga catcggaacc
     1201 aactccgtcg gatgggccgt catcaccgac gagtacaagg tcccatccaa gaagttcaag
     1261 gtcctcggaa acaccgaccg tcactccatc aagaagaacc tcatcggagc cctcctcttc
     1321 gactccggag agaccgccga ggccacccgt ctcaagcgta ccgcccgtcg tcgttacacc
     1381 cgtcgtaaga accgtatctg ctacctccaa gagatcttct ccaacgagat ggccaaggtc
     1441 gacgactcct tcttccaccg tctcgaggag tccttcctcg tcgaggagga caagaagcac
     1501 gagcgtcacc caatcttcgg aaacatcgtc gacgaggtcg cctaccacga gaagtaccca
     1561 accatctacc acctccgtaa gaagctcgtc gactccaccg acaaggccga cctccgtctc
     1621 atctacctcg ccctcgccca catgatcaag ttccgtggac acttcctcat cgagggagac
     1681 ctcaacccag acaactccga cgtcgacaag ctcttcatcc aactcgtcca aacctacaac
     1741 caactcttcg aggagaaccc aatcaacgcc tccggagtcg acgccaaggc catcctctcc
     1801 gcccgtctct ccaagtcccg tcgtctcgag aacctcatcg cccaactccc aggagagaag
     1861 aagaacggac tcttcggaaa cctcatcgcc ctctccctcg gactcacccc aaacttcaag
     1921 tccaacttcg acctcgccga ggacgccaag ctccaactct ccaaggacac ctacgacgac
     1981 gacctcgaca acctcctcgc ccaaatcgga gaccaatacg ccgacctctt cctcgccgcc
     2041 aagaacctct ccgacgccat cctcctctcc gacatcctcc gtgtcaacac cgagatcacc
     2101 aaggccccac tctccgcctc catgatcaag cgttacgacg agcaccacca agacctcacc
     2161 ctcctcaagg ccctcgtccg tcaacaactc ccagagaagt acaaggtaag tttaaacata
     2221 tatatactaa ctaaccctga ttatttaaat tttcaggaga tcttcttcga ccaatccaag
     2281 aacggatacg ccggatacat cgacggagga gcctcccaag aggagttcta caagttcatc
     2341 aagccaatcc tcgagaagat ggacggaacc gaggagctcc tcgtcaagct caaccgtgag
     2401 gacctcctcc gtaagcaacg taccttcgac aacggatcca tcccacacca aatccacctc
     2461 ggagagctcc acgccatcct ccgtcgtcaa gaggacttct acccattcct caaggacaac
     2521 cgtgagaaga tcgagaagat cctcaccttc cgtatcccat actacgtcgg accactcgcc
     2581 cgtggaaact cccgtttcgc ctggatgacc cgtaagtccg aggagaccat caccccatgg
     2641 aacttcgagg aggtcgtcga caagggagcc tccgcccaat ccttcatcga gcgtatgacc
     2701 aacttcgaca agaacctccc aaacgagaag gtcctcccaa agcactccct cctctacgag
     2761 tacttcaccg tctacaacga gctcaccaag gtcaagtacg tcaccgaggg aatgcgtaag
     2821 ccagccttcc tctccggaga gcaaaagaag gccatcgtcg acctcctctt caagaccaac
     2881 cgtaaggtca ccgtcaagca actcaaggag gactacttca agaagatcga gtgcttcgac
     2941 tccgtcgaga tctccggagt cgaggaccgt ttcaacgcct ccctcggaac ctaccacgac
     3001 ctcctcaaga tcatcaagga caaggacttc ctcgacaacg aggagaacga ggacatcctc
     3061 gaggacatcg tcctcaccct caccctcttc gaggaccgtg agatgatcga ggagcgtctc
     3121 aagacctacg cccacctctt cgacgacaag gtcatgaagc aactcaagcg tcgtcgttac
     3181 accggatggg gacgtctctc ccgtaagctc atcaacggaa tccgtgacaa gcaatccgga
     3241 aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagaccatc
     3301 ctcgacttcc tcaagtccga cggattcgcc aaccgtaact tcatgcaact catccacgac
     3361 gactccctca ccttcaagga ggacatccaa aaggcccaag tctccggaca aggagactcc
     3421 ctccacgagc acatcgccaa cctcgccgga tccccagcca tcaagaaggg aatcctccaa
     3481 accgtcaagg tcgtcgacga gctcgtcaag gtcatgggac gtcacaagcc agagaacatc
     3541 gtcatcgaga tggcccgtga gaaccaaacc acccaaaagg gacaaaagaa ctcccgtgag
     3601 cgtatgaagc gtatcgagga gggaatcaag gagctcggat cccaaatcct caaggagcac
     3661 ccagtcgaga acacccaact ccaaaacgag aagctctacc tctactacct ccaaaacgga
     3721 cgtgacatgt acgtcgacca agagctcgac atcaaccgtc tctccgacta cgacgtcgac
     3781 cacatcgtcc cacaatcctt cctcaaggac gactccatcg acaacaaggt cctcacccgt
     3841 tccgacaaga accgtggaaa gtccgacaac gtcccatccg aggaggtcgt caagaagatg
     3901 aagaactact ggcgtcaact cctcaacgcc aagctcatca cccaacgtaa gttcgacaac
     3961 ctcaccaagg ccgagcgtgg aggactctcc gagctcgaca aggccggatt catcaagcgt
     4021 caactcgtcg agacccgtca aatcaccaag cacgtcgccc aaatcctcga ctcccgtatg
     4081 aacaccaagt acgacgagaa cgacaagctc atccgtgagg tcaaggtcat caccctcaag
     4141 tccaagctcg tctccgactt ccgtaaggac ttccaattct acaaggtccg tgagatcaac
     4201 aactaccacc acgcccacga cgcctacctc aacgccgtcg tcggaaccgc cctcatcaag
     4261 aagtacccaa agctcgagtc cgagttcgtc tacggagact acaaggtcta cgacgtccgt
     4321 aagatgatcg ccaagtccga gcaagagatc ggaaaggtaa gtttaaacat gattttacta
     4381 actaactaat ctgatttaaa ttttcaggcc accgccaagt acttcttcta ctccaacatc
     4441 atgaacttct tcaagaccga gatcaccctc gccaacggag agatccgtaa gcgtccactc
     4501 atcgagacca acggagagac cggagagatc gtctgggaca agggacgtga cttcgccacc
     4561 gtccgtaagg tcctctccat gccacaagtc aacatcgtca agaagaccga ggtccaaacc
     4621 ggaggattct ccaaggagtc catcctccca aagcgtaact ccgacaagct catcgcccgt
     4681 aagaaggact gggacccaaa gaagtacgga ggattcgact ccccaaccgt cgcctactcc
     4741 gtcctcgtcg tcgccaaggt cgagaaggga aagtccaaga agctcaagtc cgtcaaggag
     4801 ctcctcggaa tcaccatcat ggagcgttcc tccttcgaga agaacccaat cgacttcctc
     4861 gaggccaagg gatacaagga ggtcaagaag gacctcatca tcaagctccc aaagtactcc
     4921 ctcttcgagc tcgagaacgg acgtaagcgt atgctcgcct ccgccggaga gctccaaaag
     4981 ggaaacgagc tcgccctccc atccaagtac gtcaacttcc tctacctcgc ctcccactac
     5041 gagaagctca agggatcccc agaggacaac gagcaaaagc aactcttcgt cgagcaacac
     5101 aagcactacc tcgacgagat catcgagcaa atctccgagt tctccaagcg tgtcatcctc
     5161 gccgacgcca acctcgacaa ggtcctctcc gcctacaaca agcaccgtga caagccaatc
     5221 cgtgagcaag ccgagaacat catccacctc ttcaccctca ccaacctcgg agccccagcc
     5281 gccttcaagt acttcgacac caccatcgac cgtaagcgtt acacctccac caaggaggtc
     5341 ctcgacgcca ccctcattca ccaatccatc accggactct acgagacccg tatcgacctc
     5401 tcccaactcg gaggagacct gcagatgtcc cgtcgtcgta aggccaaccc aaccaagctc
     5461 tccgagaacg ccaagaagct cgccaaggag gtcgagaact aggaattcat gcaagatcct
     5521 ttcaagcatt cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct
     5581 aatttatttg cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg
     5641 catctgagtg aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt tcacaatctc
     5701 tcttcaattc cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca
     5761 aatatttgta ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc
     5821 aagaaaaaag tctcattgcc taagagaaac atatgtggtt ggaaatcttc cagatcgtgg
     5881 atcactagta tgtggttgga aatcttccag atcgtggatc agagctccag cttttgttcc
     5941 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6781 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8101 ccgcgcacat ttccccgaaa agtgc
//
