LOCUS       sequence-68809-e       10710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RhopH2 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RhopH2-bio
FEATURES             Location/Qualifiers
     source          1..10710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6090..6145
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6144..6163)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8381..8399)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8467..8571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8572..8640,8641..9432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8790..8809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9603..10191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10092..10111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10345..10362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10479..10500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10515..10520,10521..10538,10539..10545)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10553..10569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10558..10580
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10577..10593
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10577..10593
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccctgg aactgaacga gaaggtgtcc atcaagtacg
     1381 gcgccgagca gggcgtgggc agcgccgaca gcaacaccaa gctgtgcagc gacatcctga
     1441 agtacctgta catggacgag tacctgagcg agggcgacaa ggccaccttc gagaagaaat
     1501 gccacaacgt gatcggcaac atccggaaca ccttcagcaa caagaacacc atcaaagagg
     1561 gcaacgagtt cctgatgagc atcctgcaca tgaagtccct gtacggcaac aacaacaata
     1621 acaacgccgg ctctgagagc gatgtgaccc tgaagtctct gtacctgagc ctgaagggca
     1681 gccagaacac cgagggcgag agcgaggtgc ccagcgacga cgagatcaac aaggccatca
     1741 tgaacttcgt gaagttcaac aagtatctgc tggacaacag caacgacatc aagaaggtgc
     1801 acgactttct ggtgctgacc agccagagca acgagaatct gctgcccaac aaagagaagc
     1861 tgttcgagca gatcgtggac cagatcaagt acttcgacga gtacttcttc gccagcggcg
     1921 gcaagatcaa agtgaagaag ggctacctga agtacaactt cctggacatc tacaagcagc
     1981 ccgtgtgcag cgcctatctg cacctgtgca gccggtacta cgagagcgtg tccatctaca
     2041 tccggctgaa gaaggtgttc aacggcatcc ccgcctttct ggacaagaac tgccggaaag
     2101 tgaagggcga ggaattcaag aaactgatgg acatggaact gaagcacaac cacatcgtgg
     2161 agcggttcga caagtacatc atcagcgacg acctgtacta cgtgaacatg aaggtgttcg
     2221 acctgaagaa cgtggacaag atccaggtgt ccaagatcga cgacatcaat aacctgaaca
     2281 tctacgagca caaagaaacc atgcacctga gcgccaagaa cctggcccgg tacatcgata
     2341 tcaagaaaga gctgaacgac gagaaggcct acaaacagct gatgagcgcc atccggaaat
     2401 acgtgaccac cctgaccaag gccgacagcg acatcaccta ctttgtgaag cagctggacg
     2461 acgaggaaat cgagcggttc ctgatcgacc tgaacttctt cctgtacaac ggcttcctgc
     2521 ggatcaccga ggacaagcac ctgatcaacg ccgacgacgt gtcccccagc tacatcaacc
     2581 tgtaccggtc caacaacatc gtggccctgt acatcctgaa aacccagtac gaggaaaaca
     2641 agctgtccga gtaccgggcc cacaagttct accggcggaa gcgggtgtcc aatatcgcca
     2701 acgacatgat caagaaggac ttcacccaga ccaacgccct gaccaatctg cccaacctgg
     2761 acaacaagaa aaccaccgag tactacctga aagaatacga gaacttcgtg gagaacttcc
     2821 agcccgatct gcacgatatc atgaagctgc agctgttctt caccatggcc ttcaaggact
     2881 gcaacgtgaa ccagaacttc gccgagacaa gcaagaagct gtggttcgat ctgctgtacg
     2941 cctacgacaa gttcggctgg ttctacatcc accccaacga agtgatcaac agcatcaata
     3001 aggccgactt cgtgcggcac gtgctggtgt cccggaactt cctgctgaag aacaacgacc
     3061 agctgacctt cctggaaacc caggtggcca aaatcgtgga gatcatcaac ctggccctgg
     3121 aagtggacaa gagccccgac agcctggact tcagcatccc catgaacttt ttcaaccaca
     3181 agaacggcta ccacgtgatg aacgacgaca agctgaagct gctgacctcc tacgagtaca
     3241 tcgacagcat tgccaacaac tactttttcc tgagcgagta caagaacgac gtgttccgga
     3301 ccggcaacaa cttcaagctg tacttcaatc tgcctaacat ctacagcctg gcctaccagc
     3361 tgttcaacga gctggccatc aacatcaacg tgatcaccaa cgtgcccctg aagaagtatc
     3421 tgaagtataa cgccgcctac gcctacttca ccctgatgaa catgatcggc aagaaccacg
     3481 acatctactc caagggcagc agattcgtgt acgcctccta catcctgggc ctggtgttct
     3541 tcatcgagag ccacatcgac attgcccggc tgaagcccaa ggacttcttc ttcatgaagc
     3601 agagcctgcc catcatcgac cacgtgtacc acaaggatct gaaaacactg aagaagaact
     3661 gcgccctgct gaccgacttc atgaagatca ataagaacag ccagaactac gccctgacac
     3721 acaccgagga aatgatcaag atcctggggc tgctgaccgt gaccctgtgg gccaaagagg
     3781 ggaagaagtc cgtctactac gacgacgatg tgtccctgta ccggaagctg atggtgtcct
     3841 gcgtgttcaa tggcggcgag acaatccagg aaaagctggc caacaatatc gagaagtcct
     3901 gcgacatcag ccagtacggc atcaagagca agaacctgaa ggatatgatc gacatcaatc
     3961 tggccatcca caagtggaac cccgccgaga tcgagaagct ggcctacagc ttcgtgctgt
     4021 cctgcaagat gcagaaactc atgtacaagc ccatgaacgt ggagaagctg cccctggaag
     4081 attactacaa gctgtctctg gcccccgaca tggtcaagac ctaccactgc tacaagctgg
     4141 gcaagcaggc cgccgagctg ctggaaagca tcatcctgaa gaaaaagttc gtgcggttca
     4201 gagtgaccga cgccatcgac gtgtacgact ttttctacat caaaaaagtg ctgtccagcc
     4261 ggatcaaaaa agagtacaac gagtttctgc aggacaagcg ggccttcgag aaaaaagagc
     4321 tggaaaccat cctgaacaac gcccccttca gcgaggaaca gaccatgaag ctgatcaata
     4381 gctacgagtg ccactggttc accagctacg agaatttccg gatcctgtgg atgcacgcca
     4441 gcagcaatct gggcaccggc acctacctca agaatttctt ctctgagctg tggcagaaca
     4501 tccggtttct gttcaagtcc aagctgaaga tccgggacat ggaatacttc agcggcgaca
     4561 tctcccagat gaatctgctg gattactaca gccccatggt gcacagcgag agccactgcc
     4621 aggaaaagat gcaagtcctg ttcatcaccc tgcgggacag caaagaggaa aaccgggccg
     4681 agatcgccca gaaagtgaag tctgcctact accagtgcaa gctggactac tacaagaacc
     4741 accacagcga cttcatccac cggatccacc ctaacgattt tctgaacaac aaggtgtacg
     4801 tgctgaagca gccctactac ctgatgtcca atgtgcctct gaacaacccc aagaaagtgt
     4861 cccggctgtt cgtgaccgag ggcaccctgg aatatctgct gctggataag atcaacatcc
     4921 ccgagtgctt cggcccctgc accaagctgc actttaacaa ggtggtcatc aaagagtcca
     4981 agcagcggat ctacgacatg accatcaaca acgccctggt gcccgagatc cagccctaca
     5041 acagacggaa gtacatgaca atctacatca acgaggccta catcaagaac atcgtgtccg
     5101 atgccctgac cagcgaagag atcaagcggc acgatatcca gaagggcaac atcaagatct
     5161 gcatgggcaa gagcacatac ctgaccgagc ccatcctgac agaggaacac tttaacctgg
     5221 cccacaagcc cgtgtacgat ttcagcagcg tgaagcacaa tctgaaagtg ttccacatga
     5281 agaatgagca tctggtgtct gaggacccca acgacgactg cttcatcaac taccccctgg
     5341 ccaccattaa cctggacatc agcgacccct acaaagagat ctccgaggac ctgatcaaga
     5401 atctgtacat tctgaagtcc agcggcgcgc cgtcgacctc catcacggcc tataagagtg
     5461 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     5521 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     5581 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     5641 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     5701 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     5761 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     5821 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     5881 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     5941 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccgga tcactgcatc
     6001 atattctgga tgcacagaaa atggtgtgga atcatcgtta agtctagaca cgtgattaat
     6061 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     6121 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     6181 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     6241 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     6301 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     6361 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     6421 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     6481 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     6541 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     6601 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     6661 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     6721 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     6781 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     6841 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     6901 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     6961 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     7021 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     7081 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     7141 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     7201 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     7261 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     7321 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     7381 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     7441 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     7501 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     7561 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     7621 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     7681 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     7741 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     7801 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     7861 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     7921 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     7981 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     8041 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     8101 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     8161 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     8221 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     8281 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     8341 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     8401 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8461 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8521 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8581 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8641 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8701 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8761 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     8821 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8881 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8941 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9001 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9061 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     9121 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9181 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9241 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9301 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9361 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9421 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9541 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9601 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9661 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9721 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9781 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9841 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9901 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9961 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10021 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10081 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10141 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10201 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10261 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10321 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    10381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    10441 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    10501 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    10561 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
    10621 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
    10681 tgctgcaggc gcagaactgg taggtatgga
//
