LOCUS       sequence-68810-e        9252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RhopH3 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RhopH3-bio
FEATURES             Location/Qualifiers
     source          1..9252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4632..4687
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4686..4705)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6923..6941)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7009..7113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7114..7182,7183..7974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7332..7351)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8145..8733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8634..8653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8887..8904
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9021..9042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9057..9062,9063..9080,9081..9087)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9095..9111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9100..9122
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9119..9135
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9119..9135
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaagg acgtgttcgc cggcttcgtg accaagaagc
     1381 tgaaaaccct gctggactgc aacttcgccc tgtactacaa cttcaagggc aacggccccg
     1441 atgccggcag cttcctggac ttcgtggacg agcccgagca gttctattgg ttcgtggagc
     1501 acttcctgag cgtgaagttc cgggtgccca agcacctgaa ggacaagaac atccacaact
     1561 ttgccccctg cctgaacaga gcctgggtgt ccgagttcct gaaagagtac gaggaaccct
     1621 tcgtgaaccc cgtgatgaag ttcctggaca aagagcagcg gctgttcttc acctacaact
     1681 tcggcgacgt ggagccccag ggcaagtaca cctacttccc cgtgaaagag ttccacaagt
     1741 actgcatcct gccccccctg atcaagacca acatcaagga cggcgagagc ggcgagtttc
     1801 tgaagtacca gctgaacaaa gaggaataca aagtctttct gagcagcgtg ggcagccaga
     1861 tgaccgccat caagaacctg tacagcaccg tggaggacga gcagcggaag cagctgctga
     1921 aagtgatcat cgagaacgag gccaccaacg acatcagcgt gcagtgcccc acctacaaca
     1981 tcaagctgca ctacaccaaa gagtgcgcca acagcaacaa catcctgaag tgcatcgacg
     2041 agtttctgcg caagacatgc gagaagaaaa ccgagagcaa gcaccccagc gccgatctgt
     2101 gcgagcatct gcagtttctg ttcgagagcc tgaagaaccc ctacctggac aacttcaaaa
     2161 agttcatgac caacagcgac ttcacactga tcaagcccca gagcgtgtgg aacgtgccca
     2221 tcttcgacat ctacaagccc aagaactacc tggactccgt gcagaacctg gacaccgagt
     2281 gcttcaagaa actgaacagc aagaacctga tcttcctgag cttccacgac gacatcccca
     2341 acaaccccta ctacaacgtg gagctgcagg aaatcgtcaa gctgtccacc tacacctaca
     2401 gcatcttcga taagctgtac aacttcttct tcgtgttcaa gaagtctggc gcccctatca
     2461 gccccgtgtc cgtgaaagaa ctgagccaca atatcgccga cttcagcttc aaagaggaca
     2521 acagcgagat ccagtgccag aacgtgcgga agtccctgga cctggaagtg gacgtggaga
     2581 caatgaaggg cattgccgcc gagaagctgt gcaagatcat cgaaaagttc atcctgacca
     2641 aggacgacgc cagcaagccc gagaagtccg acatccaccg gggcttccgg atcctgtgca
     2701 tcctgatcag cacccacgtg gaggcctaca atatcgtgcg gcagctgctc aacatggaaa
     2761 gcatgatcag cctgacccgg tacaccagcc tgtacatcca caagttcttc aagagcgtga
     2821 ccctgctgaa gggcaacttc ctgtacaaga acaacaaggc catccgctac tctagagcct
     2881 gcagcaaggc cagcctgcac gtgcccagcg tgctgtaccg gcggaacatc tacatccccg
     2941 agacatttct gagcctgtac ctgggcctga gcaacctggt gtccagcaac cccagcagcc
     3001 ccttcttcga gtacgccatc atcgagttcc tcgtgaccta ctacaataag ggcagcgaga
     3061 agttcgtgct gtacttcatc agcatcatct ccgtgctgta catcaacgag tactactacg
     3121 agcagctgtc ctgcttctac cccaaagagt tcgagctgat caagagccgg atgatccacc
     3181 ccaacatcgt ggaccggatc ctgaagggaa tcgacaacct gatgaagtcc accagatacg
     3241 acaagatgcg gaccatgtac ctggactttg agagcagcga catcttcagc cgggagaagg
     3301 tgttcaccgc cctgtacaat ttcgacagct tcatcaagac aaatgagcag ctgaagaaga
     3361 agaacctgga agagatctcc gagatccccg tgcagctgga aaccagcaac gacggcatcg
     3421 gctaccggaa gcaggacgtg ctgtacgaga cagacaagcc tcagaccatg gatgaggcca
     3481 gctacgagga aaccgtggac gaggacgccc accacgtgaa cgagaagcag cacagcgccc
     3541 actttctgga cgccattgcc gagaaggaca tcctggaaga aaagaccaag gaccaggacc
     3601 tggaaatcga gctgtacaag tacatgggcc ccctgaaaga gcagagcaag agcaccagcg
     3661 ccgccagcac ctctgatgag atcagcggca gcgagggccc tagcaccgag agcacctcca
     3721 ccggcaacca gggcgaggac aagaccaccg acaacaccta caaagagatg gaagaactcg
     3781 aggaagccga gggcaccagc aacctgaaga agggcctgga attctacaag agcagcctga
     3841 agctggacca gctggataaa gagaagccca aaaagaagaa gtccaagcgg aagaagaagc
     3901 gcgatagctc tagcgacaga atcctgctgg aagagagcaa gaccttcacc agcgagaacg
     3961 agctgggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     4021 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     4081 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     4141 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     4201 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     4261 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     4321 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     4381 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     4441 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     4501 tccaggtttt atccaaaggg ttgaattccg gatcactgca tcatattctg gatgcacaga
     4561 aaatggtgtg gaatcatcgt taagtctaga cacgtgatta attaaggatc ccccgacctc
     4621 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     4681 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     4741 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     4801 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     4861 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     4921 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     4981 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     5041 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     5101 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     5161 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     5221 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     5281 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     5341 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     5401 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     5461 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     5521 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     5581 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     5641 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     5701 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     5761 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     5821 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     5881 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     5941 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     6001 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     6061 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     6121 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     6181 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     6241 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     6301 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     6361 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     6421 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     6481 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     6541 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     6601 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     6661 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     6721 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     6781 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     6841 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     6901 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6961 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7021 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7081 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7141 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7201 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7261 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7321 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     7381 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7441 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7501 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7561 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7621 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     7681 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7741 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7801 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7861 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7921 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7981 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8041 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8281 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8941 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9001 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     9061 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9121 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
     9181 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     9241 ggtaggtatg ga
//
