LOCUS       sequence-68814-e        7320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length SPATR with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: SPATR-bio
FEATURES             Location/Qualifiers
     source          1..7320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2700..2755
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2754..2773)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4991..5009)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5077..5181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5182..5250,5251..6042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5400..5419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6213..6801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6702..6721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6955..6972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7089..7110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7125..7130,7131..7148,7149..7155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7163..7179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7168..7190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7187..7203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7187..7203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgagt tcaagcggaa gcagaaggtg gagatcaaca
     1381 gcctgcagac caacaagaac aacgacaaca tccgggagga aaagatcaag ggcgacgtgg
     1441 agagccagcc cgacgtggac ggcgacacct gtgtgatctt cagcagcagc gagggcaaca
     1501 gcagaaactg ctggtgccct agaggctata tcctgtgcag cgaagaggac gtgctggacg
     1561 tgcagggcaa gctgaacgag atcaagaaca agcacgagcg gagcctggtc acccccctgt
     1621 ggatgaagcg gctgtgcgac aacagcaacg acgtgggctt caagagcatg agcgtggtca
     1681 tcgactacga actcgctgtg ctgtgtaagg acggcagcaa caaggactac gccgacttcg
     1741 agatcatcgg cgccagcggc tatatcactg gcgaggaaat gatcgaagaa cagaagcgga
     1801 acccttggta cgtgccccgg aagtgcaccg tgaacaactt ctacctgtgc cgcaaggtgg
     1861 agaacgacaa cgtgaactgc gcctacaccc cttggagcga ttggagcgcc tgcaagaaca
     1921 atgcccagaa acggtacaga aaagtgcggc ggagcaacca gaacaatgag aacttctgcc
     1981 tgtggaacga caagatcgtg ccccgcaaca tcatggaaca gaccagaagc tgcggcgcgc
     2041 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     2101 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     2161 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2221 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2281 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2341 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2401 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2461 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     2521 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     2581 ccaaagggtt gaattccgga tcactgcatc atattctgga tgcacagaaa atggtgtgga
     2641 atcatcgtta agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     2701 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     2761 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     2821 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     2881 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     2941 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     3001 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     3061 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     3121 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     3181 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     3241 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     3301 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     3361 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     3421 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     3481 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     3541 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     3601 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     3661 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     3721 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     3781 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     3841 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     3901 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     3961 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     4021 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     4081 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     4141 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     4201 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     4261 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     4321 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     4381 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     4441 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4501 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4561 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     4621 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     4681 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4741 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4801 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     4861 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     4921 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     4981 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5041 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5101 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5161 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5221 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5281 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5341 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5401 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     5461 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5521 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5581 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5641 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5701 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     5761 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5821 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5881 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5941 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6001 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6061 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6121 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6181 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6241 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6301 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6361 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6421 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6481 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6541 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6601 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6661 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6721 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6781 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6841 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6901 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6961 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7021 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7081 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7141 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7201 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     7261 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
//
