LOCUS       sequence-68815-e        7155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length AARP ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: AARP-bio
FEATURES             Location/Qualifiers
     source          1..7155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2535..2590
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2589..2608)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4826..4844)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4912..5016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5017..5085,5086..5877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5235..5254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6048..6636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6537..6556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6790..6807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6924..6945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6960..6965,6966..6983,6984..6990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6998..7014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7003..7025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7022..7038
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7022..7038
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaaga gcatcctgcg gaacaacaag gcccacaaca
     1381 atctgcccat cagcaagacc aacgaggaag aggaaggcaa gatcaacatc aacaacctga
     1441 agcccatcaa gcagcacgac aacatcatcg aggacgtgca catcaaagag aacaagttca
     1501 tcagcatcaa gaacaaggac aagaacggcg ccttcatcga cctgagcatg cggtacaacg
     1561 agaaagagag cgacaacgac gaggaagaag aagaggacga ggaagataac gaggacaaca
     1621 acgccaacaa taacaataat aacaacaaca acaataacaa cgacgatgac caccacaacg
     1681 atgacgatca ccacgacaac aacgacaatc acgacaacga taataatgat aacaacaata
     1741 ataacaatga taataacaat aacaataatg acaacaataa taacaacaac gataacaaca
     1801 acaacgccgc tgccgccttt acagccctgc cccctcctcc tccacctgtg cctcccccac
     1861 ctcctccagg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     1921 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     1981 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     2041 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     2101 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     2161 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     2221 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     2281 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     2341 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     2401 agatccaggt tttatccaaa gggttgaatt ccggatcact gcatcatatt ctggatgcac
     2461 agaaaatggt gtggaatcat cgttaagtct agacacgtga ttaattaagg atcccccgac
     2521 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2581 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     2641 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     2701 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     2761 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     2821 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     2881 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     2941 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     3001 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     3061 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     3121 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     3181 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     3241 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     3301 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     3361 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     3421 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     3481 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     3541 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     3601 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     3661 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     3721 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     3781 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     3841 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     3901 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     3961 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     4021 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     4081 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     4141 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     4201 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     4261 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4321 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     4381 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     4441 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     4501 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     4561 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     4621 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     4681 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     4741 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     4801 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4861 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4981 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5041 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5101 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5161 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5221 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5281 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5341 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5401 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5461 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5521 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5581 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5641 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5701 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5761 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5821 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5881 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5941 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6061 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6121 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6181 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6241 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6301 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6361 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6421 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6481 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6541 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6601 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6661 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6721 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6781 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6841 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6901 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     6961 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7021 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     7081 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     7141 actggtaggt atgga
//
