LOCUS       sequence-68822-e        9099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RAMA ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RAMA-bio
FEATURES             Location/Qualifiers
     source          1..9099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4479..4534
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4533..4552)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6770..6788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6856..6960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6961..7029,7030..7821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7179..7198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7992..8580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8481..8500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8734..8751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8868..8889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8904..8909,8910..8927,8928..8934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8942..8958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8947..8969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8966..8982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8966..8982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcctacc tggaacagat caagaacggc atcagcggcc
     1381 actacaccga ggaccacaac atcaagaaca acaactgcat cagcttcagc gactacgagc
     1441 ggagcatcaa gaacttcgcc atcagcagcc acgccgagaa caactacgac aacatcatca
     1501 acgagtacaa gaagatcaag gacatcaaca acaatatcaa catcctgagc agcgtgcacc
     1561 ggaagggccg gatcctgtac gacagcttcc tggaaatcaa caagctggaa aacgacaaga
     1621 aagagaagca cgagaaagag gacgagtacg aggacaacga cgagagcttt ctggaaaccg
     1681 aggaatacga ggataacgag gacgagaagt acaacaagga cgaggacgac tacgccgaga
     1741 gcttcatcga gacagacgag tatgaggaca atgaagatga caaatataac aaagatgaag
     1801 atgattacag cgagtccttc atcgaaaccg atgagtacga cgacaatgaa gaggaacagt
     1861 ataacaagga tgaagatgac tatgctgact cctttatcga gactgaccac tacgagaaca
     1921 acgatgacaa gaacgaggaa gaggaagaat acaacgacca ggacaacgat tacggctaca
     1981 atttcctgga aacagacgaa tatgacgaca gcgaagagta cgactacgac gataaagagt
     2041 acggcgagtc ttttctggaa aaagaagagg gcgaggaaat gaaggacgaa gagatgaagg
     2101 atgaagaaat ggaagatgtg gagatgaaag acgaggaaat gaaggacgag gaaatgaaat
     2161 acgacgagat gaaaaatgaa gagatgaaat atgacgaaat gaaagatgaa gtcatgaaag
     2221 acgaggagat gaaagacgag cagatgaagt acgaagaatt caagaacgaa gagtttaaga
     2281 atgaggaatt caaaaacgag gaaagcaaaa acgaagaatc caaaaatgag gaatctaaga
     2341 atgaagaaag caagaatgaa gagttcaaaa atgaagagtc caagaacgag gaattcaaga
     2401 atgaagagtt caagaacgaa gatatgagct acgacgagta catgggctac aagaagaaag
     2461 aagaggacga atcctacaac accttcaacg gcgccaagaa gaacaacgcc gccaacagct
     2521 tcctcgagaa ggatctgcag ggcgacagcg acgatgagct gcacagcacc ttctacagca
     2581 agaacgtgga caaagaaaac tatgacgaca agaacatctt ctacggctac agcgacaatg
     2641 acgacgagtc ctttctcgag acagatagct acgaagaata tgaggatgag gacaaggacg
     2701 tggaggacga atacgaagag tccttcctgc agaacgacga gaaaaagatg gtgttctacg
     2761 acctgtacaa gcccgaagag aacgaggcct actacgagaa gaagcagaaa aaagaagaga
     2821 aagaggaaaa agaagaaaaa gagcagagcc tgaacaagca gaacgacatg gaagatcagg
     2881 aagataacga agagtacaag ttcgaggaag agaacaaaga ggatctcctc gacgtccagc
     2941 aggatgagga actgcccagc gagggcaagc agaaagtgaa gggcaagagc ttcgacaacg
     3001 agcacctgaa cgagatccag aacgtggccg acgtgcacgc cttcatccag aaagacatga
     3061 agtacctgga cgacctgatc gacgaggaac agaccatcaa ggacgccgtg aagaagtccg
     3121 cctacaaggg caacaagaag ctgggcaaca acaagaagtc ccagatcatc ctggaagagg
     3181 aacccgacga gaacttcgaa gaggacgctg acgaggaact gaacaagctg atggaacagg
     3241 aaaagaacat cgtcgacaaa gagatcaaga atagcaaggc caacaaagcc aataagaagc
     3301 tgcagttcaa caacgccaac aaacagaaca agatgtacat gaagaatgag tacaacaaca
     3361 aggccaagaa caataagaat aacaagtttg agcagcagaa ctacgatgag agctacatgg
     3421 acgacgatta cgagcagaat gaagaattca acgacaacaa ccaggccgag gacatgaaag
     3481 agacaaacga gctggacaag atcaacgacg agctgctgac cgaccagggc cccaacgagg
     3541 acaccctgct ggaaaacaat aacaagatct tcgataacaa gttcgtggcc cacaagaagc
     3601 gggagaagtc catcagcccc cacagctacc agaaagtgtc caccaaggtg cagaacaaag
     3661 aaaacatgga aaacaaagag aagaaacagc tgatcaacga tgaggccgcc atgaccgccg
     3721 aggaactggt ggagctggaa aataccgagg acgtgaacac ccctaccatg gtcgagacag
     3781 aggaaatcga cttcgacgag aacggcaaca agggcgcgcc gtcgacctcc atcacggcct
     3841 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     3901 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     3961 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     4021 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     4081 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     4141 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     4201 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     4261 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     4321 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggat
     4381 cactgcatca tattctggat gcacagaaaa tggtgtggaa tcatcgttaa gtctagacac
     4441 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     4501 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     4561 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     4621 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     4681 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     4741 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     4801 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     4861 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     4921 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     4981 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     5041 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     5101 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     5161 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     5221 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     5281 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     5341 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     5401 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     5461 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     5521 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     5581 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     5641 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     5701 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     5761 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     5821 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     5881 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     5941 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     6001 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     6061 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     6121 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     6181 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     6241 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     6301 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     6361 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     6421 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     6481 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     6541 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     6601 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     6661 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     6721 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     6781 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6841 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7081 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7141 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7201 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7261 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7321 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7381 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7441 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7501 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7561 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7621 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7681 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7741 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7801 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8101 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     8821 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8881 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8941 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     9001 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     9061 tgttgccatt gctgcaggcg cagaactggt aggtatgga
//
