LOCUS       sequence-68823-e       10482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length TLP ectodomain 
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: TLP-bio
FEATURES             Location/Qualifiers
     source          1..10482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5862..5917
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5916..5935)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8153..8171)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8239..8343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8344..8412,8413..9204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8562..8581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9375..9963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9864..9883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10117..10134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10251..10272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10287..10292,10293..10310,10311..10317)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10325..10341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10330..10352
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10349..10365
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10349..10365
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgaga agctgatcag caagctgacc aagaacggcg
     1381 acctggacct ggtgctgctg tacgacatcg gcttcgtgga cgacagcgag aacgaccaca
     1441 tgaacagcct ggaaaatatc gccgagctgg gcaagaatct gctggtgcgg gacaacaaga
     1501 acatcaacct ggcctacatc acctacgacg acatcaacgt ggatctgcgg atcgagagcc
     1561 ggaacaacat cggcgtggac aacgagaaca actacgacaa ggacaacaac gccgcctaca
     1621 agttcggcag cgacaagaat atcgaggaat tccggaacgt ggtgctggac accaagctga
     1681 agtccagcta caagaacagc gcccacctga aggccctgaa ctacgtgggc ctgaagcact
     1741 tctacaacag cgagaaagaa agcgtgaaga tggtggtgat gttcatgaac accgacggcg
     1801 actacaacaa caataacaat aataacatga acaatatcgc cagcagctac agcgccgagc
     1861 tgaacctggt gcactacctg ttcgaccgga agaatatcat cctgaacatc atcacccggg
     1921 tggccttcaa gaactactgc cactacatcc agcagatcgg cagcaacacc aacgagctgc
     1981 tgaagtgcgt gctgaagaac agcttctata atatcgccgc cctgacctac gagatcatca
     2041 agtactacga cgatatcagc atcaacgcca tctgccacgg gtggagcgag tggagcccct
     2101 gcagcgtgac ctgtaacatg ggctaccact tcagcaagcg ggacagcctg ggctacatca
     2161 agaacagcaa gagcggcctg tacaagcgga agggcagaag ctgcctggaa cagcggaacc
     2221 tgatcatcca ggaatgcttc aacaccgcct gcgaccacag cctggacatc tgcgacaaca
     2281 tctacatgga catcagcatc ctgctggacg actccagcaa tatcgccctg gaaagctgga
     2341 acaagtacat catccccttc gtgcggaagt tcatcaccca cttcaacatc aacaacaacc
     2401 agatcaactt cgccctgacc acctacagca gccacaccta caactggttc aactttgcca
     2461 acgtgctgtc caaggacaag gataagctgc tgacctacct ggaattcttc aagttcaact
     2521 tcggcagctc caccaagaat atcaagcagg ccatccagta catgaacgac cacgtgctga
     2581 acaccaacta ccggcggaac gacgccaaga aaatcatgct gatcatcaac agcggcgaga
     2641 tcgacaacaa ggccgtgacc agcgtgcccg acatcatcca gcggctgaag gacacccaca
     2701 acgtccaggt ctacagcatc tgcatcaaca atcagaacgt gaagaactgc cggaagctgt
     2761 ccacccacag catggaaaag tacggcagca acttcgcctt cagcctgccc aacgccgccg
     2821 acctgagata caacgtgctg ggcatccaga agtccatctg cagaaacgtg gctggcgcct
     2881 tcagacggaa gctgcggagc ggcaacaagg acgacgagga caagaaggat gacgagtata
     2941 aagaagagga cgaatacaaa gaagaggacg agtataaaga agaggacgag tacaaagaag
     3001 aggacgaata taaagaagag gacgaataca aagaagagga cgagtacaaa gaagaagatg
     3061 aggacatgaa aaaggacgag gatatgaaga aagatgaaga tatgaagaag gatgagtaca
     3121 agaagggcta caaggactac aagaaggacc agagcaagaa cgacaataag gccgaggaac
     3181 ggaaggacga gggcaacatt gccacctcca acgagcacca cgccagcgag aaaaccgatg
     3241 acgaggacat catcacacag tacaacgagg aagatcggca cgacaacagc aatgacaaca
     3301 tcatcatcga cgacgagccc gtgggccagg acaacagcat cttcaagaac gagaacttct
     3361 tctggggcag aggcaagcac gacagcaagt acaccagcaa gctgtccatc aactccctgg
     3421 gcgatatcaa cgagtacaac gacgattaca acaattacga ctactacgat gacgacccca
     3481 ccaaccggaa caagggcttc ctgaagaaga acagcatcat tctgaagtcc ctgtacaacc
     3541 tgaacgagga ctacgtgaac aacaacatca ataacgacgc cggcagcacc agcagccact
     3601 gccggaacaa taacgccggc cacaacgaca tcccccactt taagaacatg cggaagtaca
     3661 actccgagac aaacatcccc tgcagcagca agagcaacaa ggccaccaac aagaagctgc
     3721 tgaaccggct gtacaacatg ctgttccgga agaagaagaa gtaccggatc aagaacatgg
     3781 acgcccacag caagaaaaag gtggagaagt tcatcgagaa catcaagttc agcaaagaga
     3841 ataacgacga caaccggaag atcgacgagc agaacatcga gatccagttc gacaccctgc
     3901 tggatggcat cttcgacttt ctgcaggact acgacaactc cgccctgaac gccttcaaca
     3961 atgccgcccc tgacgacgac ttcgtgtact tcagcaacac cgccccctgt ggcgtgagcc
     4021 tgatggactt ccccatctac gacatggaaa acatggaagc cgacgtgggc ggcaagagaa
     4081 acgacggcga taacaacgac gacgacgata ataatgacga tggcgacaac aatgatgacg
     4141 gcgacaataa cgatgatgat gacaacgatg acaagtccaa taataacaat aacaacaaca
     4201 acaatggcga ccccagctct aataacaaca acaataataa caacaataac ggcgatccct
     4261 ccagcaataa caataacaat aacaatggcg accctagcag caacaacaac gatgatggcg
     4321 acgccgacga gaagcggaac gactacatca gccagtacaa tctggtgctg aaggataaga
     4381 gccacaacaa caactactac atgaccaacg agcatgacga gcggaagatc aagaagcagg
     4441 aaaacatcga catcctgagc aaagaggaag ataacattgc cagcaactac aacaatgagc
     4501 tgcagcggac caagcggagc ctggacttcc ctgagatcat catcaaagag aactacgaga
     4561 accccaagga tatcatccac atcagcaaga aagaggaagc caacgaggcc aacaacgcca
     4621 agggccacat caatctgaag aacaagaaag aacacaacaa cgtggtggac aacgtgaatc
     4681 cctgcaacaa gcagaacatt aacgaagagg acaacaatat caacaatgct atcaagaccg
     4741 cccggatcgg catcttcaac aagaataaga tcaactgcag aaacaagaag gccagcaagt
     4801 tcgacaagat caagcggttc aacgacaaga ttaacaagga caatcacccc ctgatcacca
     4861 acgtggagaa caagaacgaa gagatcgacg tgctgcagaa caacgaggat aagaagcaga
     4921 agatcctgcc catcatcaag aatgagaatg ccaaagagac aggccagaag tacgaaaaca
     4981 acgacgtgta cgaggaacat aacaaggcta acattaataa caatgtggag aagctgtata
     5041 aggacaagaa ctcccaccac atcaagaatc agtacgacaa gggcaatctg gccgagaaca
     5101 acgaagtgac ccccatctac agcaaggact acgatgccga cgacgaagag tacgagcact
     5161 ggcacggcca ccagagagac aacaaagcca cccctggcgc gccgtcgacc tccatcacgg
     5221 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     5281 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     5341 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     5401 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     5461 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     5521 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     5581 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     5641 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     5701 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     5761 gatcactgca tcatattctg gatgcacaga aaatggtgtg gaatcatcgt taagtctaga
     5821 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     5881 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     5941 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     6001 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     6061 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     6121 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     6181 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     6241 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     6301 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     6361 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     6421 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     6481 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     6541 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     6601 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     6661 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     6721 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     6781 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     6841 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     6901 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     6961 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     7021 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     7081 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     7141 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     7201 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     7261 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     7321 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     7381 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     7441 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     7501 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     7561 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     7621 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     7681 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     7741 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     7801 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     7861 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     7921 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     7981 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     8041 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     8101 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     8161 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     8221 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8281 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8341 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8401 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8461 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8521 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8581 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8641 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8701 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8761 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8821 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8881 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8941 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9001 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9061 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9121 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9181 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9241 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9301 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10021 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    10081 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    10141 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    10201 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    10261 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    10321 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    10381 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
    10441 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg ga
//
