LOCUS       sequence-68824-e         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Cortez D, Wang Y, Qin J, Elledge SJ
  TITLE     Requirement of ATM-dependent phosphorylation of brca1 in the DNA 
            damage response to double-strand breaks.
  JOURNAL   Science. 1999 Nov 5. 286(5442):1162-6.
  PUBMED    10550055
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1999 Nov 5. 286(5442):1162-6."
COMMENT     Sequence Label: pcDNAFLAG-ATM-kd
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             174..197
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             201..218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ccactagtaa cggccgccag
      121 tgtgctggaa ttctgcagat atccatcaca ctggcggccg ctcgaccacc atggactaca
      181 aggacgatga cgacaagatg catcatcatc atcatcatat gagtctagta cttaatgatc
      241 tgcttatctg ctgccgtcaa ctagaacatg atagagctac agaacgaaag aaagaagttg
      301 agaaatttaa gcgcccgatt cgagatcctg aaacaattaa acatctagat cggcattcag
      361 attccaaaca aggaaaatat ttgaattggg atgctgtttt tagattttta cagaaatata
      421 ttcagaaaga aacagaatgt ctgacaatag caaaaccaac tgtatcagcc tcaacacaag
      481 cctccaggca gaaaaagatg caggaaatca gtagtttggt caaatacttc atcaaatgtg
      541 caaacagaag agcacctagg ctaaaatgtc aagaactctt aaattatatc atggatacag
      601 cgaaagattc atctaatg
//
