LOCUS       sequence-68827-e        7464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length PF3D7_0606800 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: PF3D7_0606800-bio
FEATURES             Location/Qualifiers
     source          1..7464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2844..2899
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2898..2917)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5135..5153)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5221..5325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5326..5394,5395..6186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5544..5563)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6357..6945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6846..6865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7099..7116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7233..7254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7269..7274,7275..7292,7293..7299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7307..7323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7312..7334
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7331..7347
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7331..7347
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgtgg aggaagtggt caacaacaag gccaagcggc
     1381 tgatcgacat ctaccacgcc gccgtgaaag agctgatcca gaacgaggaa ctgatcgacc
     1441 tgatcgataa gcacaacgtg gactacagcg tgatcgagag catcgagaat ctgcccaacc
     1501 tggccgacat caacgtgaag gacgacatcg acgacgtgct gtccgagatc atcaagaaga
     1561 aagaggtcaa gatcggcgcc ctgaagaaca agaactgggg catcatcggc aactacgagc
     1621 agaacccccc cgtgggcttc tggcccgacg tgatgtacat catctgggag acaatcagca
     1681 agcacatctt caacgacgag gacgccatca atatcgccta caactactac gacaacgtgt
     1741 tcgtggccct gaacgacaag gacatccaca tgaccgacaa ctactttctg agcaacagca
     1801 gactggtgga ccagagcggc aacaatctgc ctaagctgac cagcggcctg cccatcatca
     1861 agcacagcaa caagatcatg atcctgaaag agtacaacat caacaacctg gaagatctga
     1921 agtcctacat cagcaagaac gagggcctga agatcgcctg cctgaccgag gccaactgca
     1981 acgccctgaa aaacatcttc ctggacaaag tgacctacga ctacaagagc ttcagcagct
     2041 acatcgacct gagcaagagc gtgctgtcca agagccacat catcggcgtg atcagcggca
     2101 tccccttcaa cttcaacgag cacaagatca acgtgtttga cagcttcctg aaaaccggcc
     2161 acagcgccta cttcaagggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2221 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     2281 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     2341 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     2401 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     2461 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     2521 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     2581 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     2641 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     2701 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggatcactg catcatattc
     2761 tggatgcaca gaaaatggtg tggaatcatc gttaagtcta gacacgtgat taattaagga
     2821 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2881 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     2941 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     3001 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3061 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3121 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3181 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3241 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3301 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3361 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     3421 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     3481 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     3541 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     3601 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     3661 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     3721 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     3781 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     3841 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     3901 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     3961 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     4021 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4081 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4141 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4201 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4261 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4321 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4381 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     4441 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     4501 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4561 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4621 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4681 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     4741 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     4801 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     4861 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     4921 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     4981 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5041 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5101 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5161 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5341 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5401 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5461 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5521 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     5581 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5641 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5701 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5761 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5821 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     5881 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5941 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6001 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6061 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6181 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6241 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7021 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7081 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7141 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7201 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7321 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7381 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     7441 aggcgcagaa ctggtaggta tgga
//
