LOCUS       sequence-68839-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER11 (miR-RUG)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(157..269)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(293..313)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg
       61 aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccagat cttacgcgtc
      121 gccgcgtgtt taaacgcatt agtcttccaa ttgaaaaaag tgatttaatt tataccattt
      181 taattcagct ttgtaaaaat gtatcaaaga gatagcaagg tattcagttt tagtaaacaa
      241 gataattgct cctaaagtag ccccttgaat tcgtaataga tccacggaga tcaccctgaa
      301 aatacaaatt ctcagaattg tgagatctag ggataacagg gtaatccgcg gtcgacggta
      361 tcggggacca aaatggcaag taaaatagcc tgatgggata ggctctaagt ccaacgaacc
      421 agtgtcacca ctgacacaat gaggaaaacc atgtcacatc tcgcagaact ggttgccagt
      481 gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt gcgcgtcgaa
      541 tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga gctgccgcca
      601 gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt tcagcaggcg
      661 ctgaatgcgc gtaaagcgga actggaaagc gctgcactga atgcgcgtct ggcggcggaa
      721 acgattgatg tctctctgcc aggtcgtcgc attgaaaacg gcggtctgca tccggttacc
      781 cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt ggcaaccggg
      841 ccggaaatc
//
