LOCUS       sequence-68845-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER12 (miR-RUL)
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..748
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           799..892
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     892..908
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     893..909
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagccta ccggtcgcca ccatgagcga
       61 gctgatcaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
      121 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgaagat
      181 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
      241 gtacggcagc aaagccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
      301 cttccctgag ggcttcacat gggagagaat caccacatac gaagacgggg gcgtgctgac
      361 cgctacccag gacaccagct tccagaacgg ctgcatcatc tacaacgtca agatcaacgg
      421 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acacgcggct gggaggccaa
      481 caccgagatg ctgtaccccg ctgacggcgg cctgagaggc cacagccaga tggccctgaa
      541 gctcgtgggc gggggctacc tgcactgctc cttcaagacc acatacagat ccaagaaacc
      601 cgctaagaac ctcaagatgc ccggcttcca cttcgtggac cacagactgg aaagaatcaa
      661 ggaggccgac aaagagacct acgtcgagca gcacgagatg gctgtggcca agtactgcga
      721 cctccctagc aaactggggc acagatgatg cggccgcaag ccttgttaag tgctcgcttc
      781 ggcagcacat atactatgtt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca
      841 tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg ctcgaggtcg
      901 acggtatcga taagcttgac gtccatatgc tggtaccccg tg
//
