LOCUS       sequence-68860-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER12 (miR-RUL)
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        126..144
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     ncRNA           complement(222..334)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(358..378)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgtcgggcgg aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg
       61 tgaggagggg ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag atcttacgcg
      121 taatttaata cgactcacta taggattggc ctgtcaaaag gccctgatac ccgcggggcg
      181 actccacgtc ggtgtccttg acaggcgtct tccaattgaa aaaagtgatt taatttatac
      241 cattttaatt cagctttgta aaaatgtatc aaagagatag caaggtattc agttttagta
      301 aacaagataa ttgctcctaa agtagcccct tgaattcgta atagatccac ggagatcacc
      361 ctgaaaatac aaattctcag aattgtgaga tctagggata acagggtaat ccgcggtcga
      421 cggtatcggg gaccaaaatg gcaagtaaaa tagcctgatg ggataggctc taagtccaac
      481 gaaccagtgt caccactgac acaatgagga aaaccatgtc acatctcgca gaactggttg
      541 ccagtgcgaa ggcggccatt agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg
      601 tcgaatattt gggtaaaaaa gggcacttaa cccttcagat gacgaccctg cgtgagctgc
      661 cgccagaaga gc
//
