LOCUS       sequence-68865-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER13 (miR-LUP)
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    126..159
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(166..783,784..789,790..825,826..828,829..864,865..867,
                     868..903,904..912)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
       61 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
      121 gatccataac ttcgtataat gtatgctata cgaagttatg ccaccatgtc tagactggac
      181 aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg
      241 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
      301 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
      361 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
      421 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
      481 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
      541 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
      601 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
      661 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
      721 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
      781 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
      841 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
      901 ctccccgggt aaataactt
//
