LOCUS       sequence-68866-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER13 (miR-LUP)
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        124..142
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     ncRNA           complement(220..332)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(356..376)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg
       61 aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccagat cttacgcgta
      121 atttaatacg actcactata ggattggcct gtcaaaaggc cctgataccc gcggggcgac
      181 tccacgtcgg tgtccttgac aggcgtcttc caattgaaaa aagtgattta atttatacca
      241 ttttaattca gctttgtaaa aatgtatcaa agagatagca aggtattcag ttttagtaaa
      301 caagataatt gctcctaaag tagccccttg aattcgtaat agatccacgg agatcaccct
      361 gaaaatacaa attctcagaa ttgtgagatc tagggataac agggtaatcc gcggtcgacg
      421 gtatcgggga ccaaaatggc aagtaaaata gcctgatggg ataggctcta agtccaacga
      481 accagtgtca ccactgacac aatgaggaaa accatgtcac atctcgcaga actggttgcc
      541 agtgcgaagg cggccattag ccaggcgtca gatgttgccg cgttagataa tgtgcgcgtc
      601 gaatatttgg gtaaaaaagg gcacttaacc cttcagatga cgaccctgcg tgagctgccg
      661 ccagaagagc gtccggcagc tggtgcggtt atcaacgaag cgaaagagca ggttcagcag
      721 gcgctgaatg cgcgtaaagc ggaactggaa agcgctgcac tgaatgcgcg tctggcggcg
      781 gaaacgattg atgtctctct gccaggtc
//
