LOCUS       sequence-68869-e        7620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length P12p ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: P12p-bio
FEATURES             Location/Qualifiers
     source          1..7620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1581..1592
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3000..3055
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3054..3073)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5291..5309)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5377..5481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5482..5550,5551..6342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5700..5719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6513..7101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7002..7021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7255..7272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7389..7410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7425..7430,7431..7448,7449..7455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7463..7479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7468..7490
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7487..7503
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7487..7503
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcttaca agatcaacgg cgtgtgcgac ttcagcagcg
     1381 agggcctgag cctgctgccc gaggaaaagc tggacttcag cgtgtcccgg aacgtggaca
     1441 agctgagcga cgagaacaac gtgcggcact gcgtgcactt cagcaagggc ttcgagtacc
     1501 tgcggttcat ctgccccatg cggaaggaca actacgaggg catcgagatc cggcccgtgg
     1561 aatgctttga gtacatccac atcgagggca gagagcacaa gctgtccgag atcctgaagg
     1621 gcagcctgta cgagaagtcc atcaacgaca acatcatgac ccgggacgtg ttcatccccc
     1681 ccaccatcta cgaggacatg ttcttcgagt gcacctgtga caacagcctg accttcaaga
     1741 acaacatgat cggcatccgg ggcatcatga agatccacct gaagaagaac atcctgtacg
     1801 gctgcgactt cgaccacgac gagaagctga tgaagaacaa ggccgccttc accaacttct
     1861 acgacaagca gaagatcctg cccctgatcg gcaacaacaa caacgatgac gacaacaatg
     1921 acgacgataa caacaatgat aacaataata acgacaacaa caacaacaat aacaataaca
     1981 acaacaataa caataacaat aataacaaca acatcgcctg caacgtggcc atcaagaaaa
     2041 gccaggtgta cctgggcatc atctgccctg acggctacac cctgtacccc aacgactgct
     2101 tcaagaacgt gatctatgac aacaacatca tcatccccct caagaagatc atcccccacg
     2161 atatcctgta ccaccaggac aagaacaagc ggatcacctt cgccagcttc accctgaaca
     2221 tcaacgagaa cccccctggc ttcacctgtt actgcatcaa ggaccagacc aacatcaaca
     2281 accccctgat cgtgaacttc cactttagca accaggaaac cagctacgcc accggcgcgc
     2341 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     2401 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     2461 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2521 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2581 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2641 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2701 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2761 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     2821 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     2881 ccaaagggtt gaattccgga tcactgcatc atattctgga tgcacagaaa atggtgtgga
     2941 atcatcgtta agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     3001 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     3061 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     3121 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     3181 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     3241 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     3301 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     3361 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     3421 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     3481 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     3541 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     3601 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     3661 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     3721 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     3781 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     3841 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     3901 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     3961 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     4021 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     4081 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     4141 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     4201 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     4261 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     4321 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     4381 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     4441 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     4501 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     4561 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     4621 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     4681 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     4741 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4801 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4861 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     4921 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     4981 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5041 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5101 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     5161 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     5221 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     5281 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5341 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5401 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5461 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5521 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5581 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5641 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5701 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     5761 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5821 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5881 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5941 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6001 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     6061 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6121 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6181 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6241 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6301 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6361 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6421 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6481 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6541 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6601 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6661 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6721 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6781 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6841 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6901 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6961 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7021 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7081 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7141 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7201 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7261 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7321 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7381 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7441 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7501 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     7561 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
//
