LOCUS       sequence-68870-e        8547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length PF3D7_1136200 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: PF3D7_1136200-bio
FEATURES             Location/Qualifiers
     source          1..8547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3927..3982
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3981..4000)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6218..6236)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6304..6408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6409..6477,6478..7269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6627..6646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7440..8028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7929..7948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8182..8199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8316..8337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8352..8357,8358..8375,8376..8382)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8390..8406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8395..8417
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8414..8430
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8414..8430
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgctctgt acatcttcga caacgtgtgc accagcatcc
     1381 acgtgaacga cctgggctac aaggtgtccg acaccatcaa cagcttcaag tacaagaaga
     1441 actacgacaa cagcgtggtg ctggacaagg tgcccgatac ccccctggac aacaacgcca
     1501 agctgagctt ccaggaactg ctgcggaagc ggagcagcga cgacgacgat gacaacgacg
     1561 acgagggcga caacgatgat gacgatgacg acaacgacaa caacaacaac aataataaca
     1621 acaacaacaa tatgaaccgg ttctacaaca tggccagcat ccagcctgcc gccttcaaca
     1681 atatcgccca gaacggcaac caggccttca ccgcccggat gaagcagaac ctggccaagt
     1741 acaacccctt caagaagtcc ggcagcaaca acaacggcaa agtgaacaat gccaacgaga
     1801 acgtggacga ggacaacaat gacaatgtgg acgacaacaa tgctaagaag aagcaccggc
     1861 acatcaagca caagagccag ggcaccctga gcaacgacgg ctccaacaac atgaacaaca
     1921 acaacgtcaa caacaacaat gtgaacaaca acaacgtcaa caacaacaat gtcaacaaca
     1981 acaacgtgaa caacaacaat gtgaacaaca acaacatgat taacaacaac atgaacaaca
     2041 acaatatgaa caacaataac atgaacaaca acaacatgaa caatatcaat gtcaacaatg
     2101 ccaacatgaa caacaatatc aacaacaaca tgaacaacaa caacaacgtg aacgccgcca
     2161 acatcctgct gggcgccagc gctctgacag gcgccgctat cagcggccag aaccagaatg
     2221 gcatcaacaa caatcagaac gtggtcaaca acaatgcaaa caatggcgcc atccagaaca
     2281 gcatcatgct gaacaacgcc gcctccatct tcagcaacct gttcgagaac aacaacaagt
     2341 tcaataccgc cgagatgatc tgcagcagca tcaactgcgc cagcatcaac agccagaaca
     2401 acgacaagat ccccctgacc gactgcagca acgtgctgta ctgcggcaac tgccccttca
     2461 gcagcagccc caaggaccag tgcgcctcca tcaagaccct gaacgtggac aacacctata
     2521 tcgccagcgg ctacgtggac agcctgcagc tgctgaacaa gaacaacctg gtcaacatcc
     2581 ccttcacctg ggacaagacc accctggact tcagcaagtg cctgcccgac ctgaacaagt
     2641 ttaagctggc caccaaggac ctgcccaaca aggacaagaa cgaggtgtcc aagcggctgt
     2701 tccggctggc caacctgtac ctgtacacca ccaagttcgt cgaggataac aacaacaatc
     2761 ccctgaagat cagcatcaat ttcaacaaca ttaacatcga cagcctgaag cccaccgagc
     2821 agctggccaa cgagtgcaac agcctgctgc cctgccccct gaatatcgcc gagatcaaga
     2881 cctacaacat ccacgccacc cagagcctgg tgctgtacca gagactgttc atccccgaga
     2941 tctacaacgc cctgaacacc gccaatatca tgatcaatat cgcctccgac cccggctaca
     3001 acgtggccaa ctacttcttc tacgtgaaga tcgactgcgc caagaccaac gacatcatcg
     3061 agaacgagat ctaccccacc gccaccctgc cccagaccaa ccagcagacc acacagcagc
     3121 tgacccagca gacaatcccc cagaccccca tcgagaacaa tccccagcag aacgaggcca
     3181 agaatggcgc ccctcccaag aacgacgcca gcgtccagct gctcaagaac aatgccgcca
     3241 gcaaggacag cagacgggga ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     3301 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     3361 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     3421 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     3481 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     3541 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     3601 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     3661 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     3721 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     3781 agatggactc caagatccag gttttatcca aagggttgaa ttccggatca ctgcatcata
     3841 ttctggatgc acagaaaatg gtgtggaatc atcgttaagt ctagacacgt gattaattaa
     3901 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     3961 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     4021 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     4081 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     4141 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     4201 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     4261 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     4321 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     4381 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     4441 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     4501 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     4561 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     4621 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     4681 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     4741 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     4801 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     4861 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     4921 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     4981 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     5041 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     5101 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     5161 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     5221 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     5281 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     5341 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     5401 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     5461 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     5521 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     5581 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     5641 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5701 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5761 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5821 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     5881 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     5941 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     6001 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     6061 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6121 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     6181 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     6241 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6421 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6481 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6541 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6601 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6661 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6721 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6781 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6841 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6901 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     6961 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7021 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7081 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7141 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7201 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7261 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7321 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7561 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8221 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8281 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8341 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8401 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     8461 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     8521 tgcaggcgca gaactggtag gtatgga
//
