LOCUS       sequence-68871-e        9465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length PF3D7_1431400 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: PF3D7_1431400-bio
FEATURES             Location/Qualifiers
     source          1..9465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4845..4900
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4899..4918)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7136..7154)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7222..7326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7327..7395,7396..8187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7545..7564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8358..8946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8847..8866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9100..9117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9234..9255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9270..9275,9276..9293,9294..9300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9308..9324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9313..9335
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9332..9348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9332..9348
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgctaaca aggccaccaa cgtggccaac aacttcgcca
     1381 gcaatatcgc caccaatctg gccagcagcc tggccacccc cctgcctaca aacgtggcca
     1441 gccacctgat ccccaacctg gtggccgccc agcagaacaa tgccgcccac gtggacagcc
     1501 acaagaccat cagcaccaac aagcacacat gcgaggccgc tggctgcagc agctacaagg
     1561 acatcatcaa gaacaacgcc gccttccaca ccagcaacgg ccaccagagc aacgacggcg
     1621 actgcctgaa cggcttcatc tgcaagaagt gcaagaaaac ccacgccaag agccccaaca
     1681 tctgcttcta cagctccctg gacacctacg agaacctgta cgagggcatc ctggaagatt
     1741 tcaagcagac cagctacgac agcttcaaga tccccctgga caagagcagc aagaacatca
     1801 agagcaaggg caagcacaag aaccacaata ccaacaaaga ggacaacgac aacaacgata
     1861 acaacaatga caacaacaac gataacaaca acaacaacaa taacaataac aacaacaaca
     1921 acaaggacaa ccacaacaag aaggacacca acgagaacga ggaagatgac gaggacgacg
     1981 ataacaagaa caagaacaag ggcgacaagg acgacgaccg ggacgacgaa gaggactaca
     2041 agtccagcac caaagagaac aacctgaaga agaagaaaaa gaagtccgac gaggataacg
     2101 aggaaaccga ggacatcgac gacgagggcg aagaggacga ccagaacgaa gaggatgatg
     2161 aggataacaa cgccaaaaag aagaagttca acaacaaagc caacaacgag gccgacagct
     2221 tcctggaaaa gtacagcaag atcagcttcc acacctttct gacccccaac aagctgacct
     2281 gcaaggatat caaagagaag aaaaaatcca agaagatgaa gcagcggatc tacatcaaca
     2341 acaacaaaaa gaaccccgcc tgccccctgg aacctctgga acccagcctg atccagtaca
     2401 acatcatcaa cctcaagacc aacaagaatg aggccatcga taagaacaag cggatcgata
     2461 ttaacaacaa gggggacacc tacacctaca gcttccccaa gtacagcttc ctcaaagagg
     2521 aagaacggat ccaggaaatg gtcagcaacg agaagaacga caagaaagag gaacacgtcc
     2581 tgtaccggcg gctgaagatc aatatcaaca aatacgagga atacctgaag tccaagctga
     2641 acaagtgcca catcagcgac gacggaatcg ccaccatctt catcaagctg atcctccaga
     2701 tcgtgaagaa caaggccgag atctacaccg accccagcaa gcggagcatc ctgaacaaca
     2761 acgctgccat caacaccgcc accaccaaga acaacaataa caacaacaat gctaacaaag
     2821 agaagcacaa gtacagcgac gaagatgatg aagataacga ggacaatgag gataatgaga
     2881 acgacggcta caacagctac ggccagaaga acaaaaacaa cctggccgag gacgactacg
     2941 acaacgagaa tgacaacgac gagtacggca acaaaaacaa gaactccaac caggcctaca
     3001 actacatgag cagccagaac aacaatgatc ataacaacca ggtcaacaaa tatgcctaca
     3061 tccagctgct ggacaagaac taccagaaca tctacaacac caacaacccc atcaacaaga
     3121 tccaccagaa gaactacgcc tacgagaaga agtacaacca ggaccggaag aacaagatcc
     3181 tgagcaccca ggtgcccaat accgccaagt acaacttctt cgagtaccac aacgtggacg
     3241 agctggaagg cgacagcatg gaaaactact acaagagcaa gcggggcttc ttcaagaaca
     3301 tcttcaagaa ggtgttcaag cggaagaagg gcaaagaggg cgacgaaggc gaaggcgagg
     3361 gggaaggcga ggaagagttc cacaacgagg aagtggaatc caaggggaaa gagaaaaaga
     3421 aaaaaaagct gttcagcttc ttccggcgga aaaacaagaa gaacaaagct aagaacgagg
     3481 gggacgacag ctacaatgag gccaaccagg gcgaagataa tgaggataac tatgagaaaa
     3541 aaaagaacaa gcgctacgac atggacaacg atgagaaaga gaaagaaaac gacgacgagg
     3601 aagataacga caacaacaac aacaacgcct acagctacat ctccaacaac aagaagaaga
     3661 aaaaaaacga caagaaaaag aagaagaagt ttaacaagca caagctgaag attaagaact
     3721 tcttcaccaa gctcaagcag aaggtgctgc ccaagaagca gaagctgcac atcgaggcct
     3781 ttttcaacaa tatcatcgtg aagtcctgca agaacagcct gaagtggaag ggcaagatgt
     3841 tcaagaagcg gagcctgatc gagatgaccc tgaaggtgcc cgccaagatc aagtacatcg
     3901 aggacgagcc cctgaacttc ttccgcagcg gctacgaagt gatcctgaca tgcaagaact
     3961 gcgaggaaat cctgttcaac agctgcgtgc aggtctactg caccaagacc aagaccgaga
     4021 acgatgataa gaaaaacaaa aagcagacca acaccggcgc caccaccccc agcttcacca
     4081 caatggccag cgccgccagc ttcgcccctc tgcccgagta caactaccag cagctgtacc
     4141 ccctgaacag caactgggcc ctgagcgact acttcagtgg cgcgccgtcg acctccatca
     4201 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     4261 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     4321 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     4381 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     4441 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     4501 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     4561 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     4621 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     4681 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     4741 ccggatcact gcatcatatt ctggatgcac agaaaatggt gtggaatcat cgttaagtct
     4801 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     4861 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     4921 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     4981 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     5041 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     5101 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     5161 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     5221 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     5281 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     5341 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     5401 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     5461 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     5521 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     5581 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     5641 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     5701 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     5761 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     5821 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     5881 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     5941 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     6001 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     6061 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     6121 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     6181 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     6241 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     6301 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     6361 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     6421 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     6481 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     6541 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     6601 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     6661 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6721 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     6781 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     6841 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     6901 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     6961 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     7021 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     7081 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     7141 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7561 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7861 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9061 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9301 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     9361 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     9421 caacgttgtt gccattgctg caggcgcaga actggtaggt atgga
//
