LOCUS       sequence-68873-e        7404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSRP2 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSRP2-bio
FEATURES             Location/Qualifiers
     source          1..7404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2784..2839
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2838..2857)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5075..5093)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5161..5265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5266..5334,5335..6126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5484..5503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6297..6885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6786..6805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7039..7056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7173..7194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7209..7214,7215..7232,7233..7239)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7247..7263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7252..7274
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7271..7287
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7271..7287
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgctaaga acaacaacaa gcggaccagc tacaacaaga
     1381 agaaagagaa cctgaagaac ttcgacaaca tcgtgctgga cgacttctac agcgccttcg
     1441 atagccagga caactacgag agcaagctga agaagaacga ggacgacatc atcggccagg
     1501 gcatcttcag cctgatcagc aagaagaacc aggaaaaaga gaagtccctg aagggcgaga
     1561 gcgaggacaa caccaagctg caggtcacaa aggtgcaggg cgcccaggtg gaccaggccg
     1621 tggaacccct ggaaaagagc cccgaggacg agaacaaaga gatccccacc ctggacagcc
     1681 cccagaacgg caaccccgcc agctacacca gcaacctggc caccccccca ctgaagcgga
     1741 tggacgaggt gttcgacgac gtgctgaagc acctgaacaa agaggacaag gtcgtcacag
     1801 atgagaacaa gaacaagtac aacgagttca agaaagagtt cgacatcttc accatgaacg
     1861 tggccgagta cgagatcatg aagaacctgc tgatcacctt ctccaagaag atcgacgaaa
     1921 acaaccagat ccagaccaag atcgagaaca tcttcaacaa ggccctgaag gacaacaagt
     1981 acaaagagca gttcaagaac ttcatctacg gcctgtacag cttcgccaag cggcacaact
     2041 acctgatcgt gaacaaggcc aacgacgcca ccctgcacaa ggacctgttc gagaacgccc
     2101 tgaacctgat caacactggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2161 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     2221 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     2281 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     2341 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     2401 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     2461 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     2521 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     2581 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     2641 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggatcactg catcatattc
     2701 tggatgcaca gaaaatggtg tggaatcatc gttaagtcta gacacgtgat taattaagga
     2761 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2821 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     2881 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     2941 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3001 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3061 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3121 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3181 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3241 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3301 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     3361 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     3421 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     3481 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     3541 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     3601 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     3661 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     3721 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     3781 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     3841 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     3901 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     3961 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4021 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4081 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4141 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4201 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4261 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4321 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     4381 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     4441 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4501 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4561 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4621 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     4681 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     4741 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     4801 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     4861 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     4921 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4981 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5041 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5101 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5161 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5221 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5281 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5341 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5401 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5461 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     5521 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5581 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5641 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5701 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5761 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     5821 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5881 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5941 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6001 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6061 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6121 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6181 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6241 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6421 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6781 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6841 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6901 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6961 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7021 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7081 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7141 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7201 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7261 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7321 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     7381 aggcgcagaa ctggtaggta tgga
//
