LOCUS       sequence-68875-e        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP9 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP9-bio
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4170..4225
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4224..4243)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6461..6479)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6547..6651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6652..6720,6721..7512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6870..6889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7683..8271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8172..8191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8425..8442
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8559..8580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8595..8600,8601..8618,8619..8625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8633..8649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8638..8660
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8657..8673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8657..8673
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgctaaca agaacgacaa gaaccagggc gtggacatga
     1381 acgtgctgaa caactacgag aacctgttca agttcgtgaa gtgcgagtac tgcaacgagc
     1441 acacctacgt gaagggcaag aaggccccca gcgaccctca gtgcgccgac atcaaagagg
     1501 aatgcaaaga gctgctgaaa gagaagcagt acaccgacag cgtgacctac ctgatggacg
     1561 gcttcaagag cgccaacaac gccgccaaca atggcaagaa gaacaacgcc gaagagatga
     1621 agaacctggt caacttcctg cagagccaca agaagctgat caaggccctg aagaagaaca
     1681 tcgagagcat ccagaacaag aagcacctga tctacaagaa caaggcctac aaccccctgc
     1741 tgctgagctg cgtgaagaaa atgaacatgc tgaaagaaaa cgtggactac atccagaaga
     1801 accagaacct cttcaaagaa ctgatgaacc agaaggccac ctacagcttc gtgaacacca
     1861 agaagaagat catcagcctg aagtcccagg gccacaagaa agagacaagc cagaaccaga
     1921 atgagaacaa cgacaaccag aaataccagg aagtgaacga cgaggacgac gtgaacgatg
     1981 aagaggacac caacgacgat gaggatacca atgacgagga agatacaaac gacgacgagg
     2041 acacaaacga tgatgaggac actaatgatg aagaagatac caacgacgaa gaagatcacg
     2101 agaacaacaa cgctgccgcc tacgagctgg gcatcgtgcc cgtgaacgac gtgctgaatg
     2161 tgaacatgaa gaacatgatc accggcaaca acttcatgga cgtggtcaag aacaccctgg
     2221 cccagtctgg cggcctgggc agcaacgacc tgatcaattt cctgaaccag ggcaaagaga
     2281 tcggcgagaa cctgctgaat atcgccaaga tgaacctggg cgacaagaac aacctcgaga
     2341 gcttcccact ggacgagctg aatatgctga aggacaacct gatcaactat gagttcatcc
     2401 tggacaacct caagaccagc gtcctgaaca agctgaagga cctgctgctg cggctgctgt
     2461 ataaggccta cgtgtcctac aagaagcgga aggcccagga aaagggcctg cccgagccca
     2521 ccgtgaccaa cgaggaatac gtggaagaac tgaagaaagg catcctcgac atgggcatca
     2581 agctgctgtt cagcaaagtg aagtccctgc tgaagaagct caagaacaag atcttcccca
     2641 agaagaaaga ggacaatcag gccgtcgaca ccaagagcat ggaagaaccc aaagtgaagg
     2701 cccagcccgc cctgagaggc gtggaaccta ccgaggacag caacatcatg aacagcatca
     2761 acaacgtgat ggacgagatc gacttcttcg agaaagagct gatcgaaaac aacaatgccc
     2821 ccaacgtggt gccccccacc cagagcaaga agaaaaacaa aaacgaggcc gtgtccggca
     2881 tggacgagaa cttcgacaac caccccgaga actacttcaa agaagagtac tactacgacg
     2941 agaatgacga catggaagtg aaagtgaaaa agatcggcgt gaccctcaag aagttcgagc
     3001 ccctgaagaa cggcaacgtg gccgagacaa tcaagctgat ccacctgggc aacaaggata
     3061 agaagcacat tgaggccatc aacaatgaca tccagatcat caagcaggaa ctgcaggcca
     3121 tctacaacga gctgatgaac tacgccaacg gcaacaagaa catccagcag atcttccagc
     3181 agaacatcct ggaaaacgac gtcctcaacc aggaaaccga ggaagagatg gaaaagcagg
     3241 tcgaggccat caccaagcag atcgaagccg aggtggacgc cctggccccc aaaaacaaag
     3301 aggaagagga aaaagagaaa gaaaaagaag agaaagagaa agaggaaaaa gaaaaagaga
     3361 aagaagaaaa agaaaaagaa gagaaagaaa aggaagaaaa agagaaagaa gaaaaagagg
     3421 aagagaagaa agagaaagag gaagaacagg aagaggaaga ggaagaagaa atcgtccccg
     3481 agaacctggc caccgaggaa tctggcgcgc cgtcgacctc catcacggcc tataagagtg
     3541 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     3601 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     3661 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     3721 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     3781 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     3841 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     3901 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     3961 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     4021 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccgga tcactgcatc
     4081 atattctgga tgcacagaaa atggtgtgga atcatcgtta agtctagaca cgtgattaat
     4141 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     4201 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     4261 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     4321 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     4381 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     4441 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     4501 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     4561 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     4621 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     4681 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     4741 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     4801 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     4861 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     4921 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     4981 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     5041 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     5101 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     5161 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     5221 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     5281 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     5341 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     5401 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     5461 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     5521 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     5581 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     5641 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     5701 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     5761 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     5821 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     5881 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5941 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     6001 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     6061 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     6121 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     6181 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     6241 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     6301 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6361 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     6421 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     6481 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6601 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6661 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6721 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6781 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6841 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6901 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6961 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7021 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7081 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7141 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     7201 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7261 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7321 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7381 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7441 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7501 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7561 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7621 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7681 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7741 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7801 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7861 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7921 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7981 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8041 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8101 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8161 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8221 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8281 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8341 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8401 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8461 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8521 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8581 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8641 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     8701 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     8761 tgctgcaggc gcagaactgg taggtatgga
//
