LOCUS       sequence-68876-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER13 (miR-LUP)
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           152..245
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     245..261
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     246..262
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             283..300
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             301..324
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      334..343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
       61 atcctcataa aggccaagaa gggcggaaag atcgccgtgt aagcggccgc aagccttgtt
      121 aagtgctcgc ttcggcagca catatactat gttgaatgag gcttcagtac tttacagaat
      181 cgttgcctgc acatcttgga aacacttgct gggattactt cttcaggtta acccaacaga
      241 aggctcgagg tcgacggtat cgataagctt gacgtccata tgctggtacc ccgtggaagt
      301 ctggaagttc tcttccaggg gcccgaagga gacgccacca tgggtctaga tagggataac
      361 agggtaatgg taccggatcg atcccccaac ttccaggccc gccatagtga tttgatttgc
      421 cagcgcatcg ctatcaatcg ccgggttcac ccattcgcgt aaccacagtt cactgaattt
      481 cataatctat tcctgcctta tttaaactgt ttgaggaaac gcagatcgtt ttcgaagaat
      541 gaacgcaggt cggtgacgcc gtaacgcaac atagtcagac gctccatccc catcccgaag
      601 ccgaaaccag agtaaacttc cgggtcgatg ccaacgttac gcaacacgtt cggatgcacc
      661 atcccgcagc ccagcacttc cagccattta ccgtttttac ccatgacgtc cacttctgca
      721 gaaggttcgg taaacgggaa gtaggaagga cggaagcgaa tctgcaaatc ttcctcaaag
      781 aagttacgca ggaagtcgtg cagcgtgcct ttcaggttgg taaagctgat gttggtatca
      841 acaatcagac cttccatctg atggaacatc ggcgtgtgag tctggtcgta gtcgttac
//
