LOCUS       sequence-68877-e        8700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length GAMA ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: GAMA-bio
FEATURES             Location/Qualifiers
     source          1..8700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4080..4135
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4134..4153)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6371..6389)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6457..6561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6562..6630,6631..7422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6780..6799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7593..8181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8082..8101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8335..8352
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8469..8490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8505..8510,8511..8528,8529..8535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8543..8559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8548..8570
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8567..8583
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8567..8583
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgctctga tccggaacag caataccccc caggccttcc
     1381 tgatccccga gctgaacaac aacgagaaga acgagttcaa caacaatgag aaaaatgaga
     1441 tgaacaacaa tctgaacaat gagtttaaca acaacgaaga gaactgcgac atccagaaaa
     1501 tcgccgagga aatgatggaa aacctgctga acgagaacga catgtacacc aacatcatgc
     1561 tgagcctgca gaaccggctg agcgacgact acctgtgcag cgagcctaag tacgagaaca
     1621 tctgcatcca cgagaaggac aagatcagcc tgagcttccc ctgcagcaac cccaaatacg
     1681 agaagctgat ccacaagttc accttcaaga agctgtgcaa cagcaaggcc gccttcaaca
     1741 acgccctgct gaagtccttc atcgaagagg acgaggaaca gaacaccttc agcctgatgc
     1801 tgaagcagtt caagatcctg ctgacctgcg tggacgacga gctgaaggac atctacaaag
     1861 agtccatcga cctgctggtg gacctgaaaa ccagcatcac cgagctgacc cagaagctgt
     1921 ggtccggcaa gatggtcaac gtgctgaaga agagagagtt cctgatcacc ggcatcctgt
     1981 gcgagctgcg gaacggcaac aaggcccccc tgatcagcaa gagcctggaa ttcgagaacc
     2041 tgggcatcct gaagatgaac aatgaagaac tgctcaacga ggcctacaac gccttcagcg
     2101 actactacta cttcttccca tacttcatcc agaagctgct cgagaagggc ggcatgatcg
     2161 agcggctgat caagattcac gagaatctgg ccaagtaccg gaccaaggac atggtcaaca
     2221 agatcaacgc ccagagcaag ggcgaggtgc tgaacaacga ggacatcctg aacaagctga
     2281 acgcctacaa gcactacacc aagcacggcg ccaccagctt catccagtcc cgggaagtga
     2341 agatcgtgaa ccagaacgtg aacaacgacg ataccaccaa gaaccagcag cagaatgtca
     2401 acaacaacga aaagctgaac aacaacaaca acaataacaa caaccagcag gtcaacaaca
     2461 acaacaataa caacaaccag caggtcaaca acaacaataa caacaacaac aaccaggtca
     2521 acaacaacaa caataacaac aacaaccagg tcaacaacaa caattacaac aacaacaatc
     2581 aggtcaacaa caataacaac aacaatcagc aggtcaacaa caacaacaac tataacaatc
     2641 agctgaacaa caacaacttc aacaacaacc tccaggtcaa caagaacgac aagcacgtgc
     2701 ccaagaacaa ccacgccacc gccacccaca ccaacaacat cctgtacaac cccctgtaca
     2761 gcatcaaccc cgagaagccc aaggacatca tcaagctgct gaaggacctg atcaagtacc
     2821 tgcacatcgt gaagttcgag aacaacgagc ccaccaccaa catcgacgag gaaggcatca
     2881 gaaagctcct cgagaacagc ttcttcgacc tgaacgacga catcctcatc gtgcggctgc
     2941 tgctgaagcc ccagaccgtg atcctgacag tgatccagag cttcatgctg atgaccccca
     3001 gccccagccg ggatgccaag gcctactgca agaaggccct gattaacgac cagctggtgc
     3061 ccaccaacga cgccaacatc ctgagcgaag agaacgagct ggtcaacaac ttcgccacca
     3121 aatacgtgct gatctacgag aagatgaagc tgcaggaact gaaagagatg gaagagtcca
     3181 agctcaagat gaagtacagc aagaccaacc tggccgccct gcaggtcaca aacccccaga
     3241 acaacaagga caagaatgac gccagcaaca agaacaacaa tcccaacaat gccgccaccc
     3301 ccctgattgc cgtggtcacc gatctgagcg gcgagaaaac cgaggatatc attaacaaca
     3361 atgtggatat cgccaccctg agcgtgggcg tgcagaatac cttccaggga cctggcgcgc
     3421 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     3481 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     3541 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     3601 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     3661 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     3721 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     3781 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     3841 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     3901 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     3961 ccaaagggtt gaattccgga tcactgcatc atattctgga tgcacagaaa atggtgtgga
     4021 atcatcgtta agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     4081 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     4141 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     4201 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     4261 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     4321 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     4381 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     4441 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     4501 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     4561 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     4621 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     4681 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     4741 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     4801 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     4861 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     4921 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     4981 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     5041 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     5101 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     5161 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     5221 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     5281 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     5341 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     5401 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     5461 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     5521 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     5581 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     5641 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     5701 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     5761 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     5821 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5881 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5941 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     6001 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     6061 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6121 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6181 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     6241 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     6301 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     6361 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6421 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6541 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6781 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     6841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7081 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     7141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7441 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7501 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7561 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7621 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7681 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7741 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7801 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7921 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7981 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8041 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8101 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8161 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8221 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8281 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8341 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8401 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8461 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8521 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8581 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     8641 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
//
