LOCUS       sequence-68878-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER13 (miR-LUP)
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(118..136)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttgacctcc atagaagaca ccgggaccga tccagcctac cggtcgccac catggaagac
       61 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
      121 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
      181 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
      241 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
      301 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
      361 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct
      421 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc
      481 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
      541 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca
      601 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt
      661 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga
      721 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
      781 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtc
//
