LOCUS       sequence-68885-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing MSCV LTR promoter, dCas9 that is 
            fused to 2x NLS, VP64 and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pMSCV-LTR-dCas9-VP64-BFP
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..26)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(33..182)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(189..209)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(225..245)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatcccactt tcctcttctt tttgggacta cctagcatat ctagatcaaa gtcatctagg
       61 gcatcggagc ccaacatatc taaatcgaag tcgtccaatg catctgagcc taacatgtct
      121 aggtcgaagt cgtctaacgc gtctgaacca agcatatcta agtcaaaatc gtctaaagca
      181 tcactaccaa ctttgcgttt ctttttcgga gatccggaac tacctacctt gcgctttttc
      241 ttgggagctc cctcatcccc tccgagctgt gagaggtcaa tccgagtttc gtaaagtccg
      301 gtgatgctct ggtggatgag ggtggcgtcc agcacttctt tggtggaggt atagcgtttc
      361 cggtcgatgg tggtgtcgaa gtacttgaat gcggcagggg caccaagatt ggtgagggtg
      421 aacaggtgga tgatattctc agcctgctcc cgaattggtt tgtctctgtg cttattgtat
      481 gcgctaagga ctttatcgag gttggcatca gcgagaatga cgcgtttaga aaactcggaa
      541 atctgctcaa tgatctcgtc cagataatgc ttatgctgct ccacaaacag ttgcttttgc
      601 tcgttgtcct cggggctccc cttcagcttc tcgtaatggc ttgcaaggta gaggaaattc
      661 acgtacttgg aggggagagc cagctcatta cccttctgaa gttcgccagc ggaggcgagc
      721 atccgcttcc gcccattctc cagttcgaac aggctatact ttgggagctt gatgataagg
      781 tccttcttga cttccttgta acctttggct tcgagaaagt caattgggtt cttctcgaag
      841 gagctgcgct ccataatggt gatacccagc agttccttca cgctcttgag cttcttagac
      901 tttccttttt ccacctta
//
