LOCUS       sequence-68886-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing MSCV LTR promoter, dCas9 that is 
            fused to 2x NLS, VP64 and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pMSCV-LTR-dCas9-VP64-BFP
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..775)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(782..802)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag aattcgagac
       61 tctcgagagt ctaggatcca ttaagcttgt gccccagttt gctagggagg tcgcagtatc
      121 tggccactgc cacctcgtgc tgctcgacgt aggtctcgtt gttggcctcc ttgattcttt
      181 ccagtctgta gtccacatag tagacgccag gcatcttgag gttcttagcg ggtttcttgg
      241 atctatatgt ggtcttgatg tttgcgatca gatggctccc gcccacgagc ttcagggcca
      301 tgtcgtttct gccttccagg ccgccgtcag cggggtacag cgtctcggtg aaggcctccc
      361 agccgagtgt tttcttctgc atcacagggc cgttggatgt gaagttcacc cctctgatct
      421 tgacgttgta gatgaggcag ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc
      481 ccccgtcttc gtatgtggtg actctctccc atgtgaagcc ctcagggaag gactgcttga
      541 agaagtcggg gatgccctgg gtgtggttga tgaaggtctt gctgccgtag aggaagctag
      601 tagccaggat gtcgaaggcg aaggggagag ggccgccctc gaccaccttg attctcatgg
      661 tctgggtgcc ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tgatggttgt
      721 ccacggtgcc ctccatgtac agcttcatgt gcatgttctc cttaatcagc tcgctagatc
      781 ccactttcct cttctttttg ggactaccta gcatatctag atcaaagtca tctagggcat
      841 cggagcccaa catatctaaa tcgaagtcgt ccaatgcatc tgagcctaac atgtctaggt
      901 cgaa
//
