LOCUS       sequence-68903-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS 
            controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..86)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tatttaataa taaaaatcat aaatcataag aaattcgcct cgagagtcta ggatccggaa
       61 ctacctacct tgcgcttttt cttgggagct ccctcatccc ctccgagctg tgagaggtca
      121 atccgagttt cgtaaagtcc ggtgatgctc tggtggatga gggtggcgtc cagcacttct
      181 ttggtggagg tatagcgttt ccggtcgatg gtggtgtcga agtacttgaa tgcggcaggg
      241 gcaccaagat tggtgagggt gaacaggtgg atgatattct cagcctgctc ccgaattggt
      301 ttgtctctgt gcttattgta tgcgctaagg actttatcga ggttggcatc agcgagaatg
      361 acgcgtttag aaaactcgga aatctgctca atgatctcgt ccagataatg cttatgctgc
      421 tccacaaaca gttgcttttg ctcgttgtcc tcggggctcc ccttcagctt ctcgtaatgg
      481 cttgcaaggt agaggaaatt cacgtacttg gaggggagag ccagctcatt acccttctga
      541 agttcgccag cggaggcgag catccgcttc cgcccattct ccagttcgaa caggctatac
      601 tttgggagct tgatgataag gtccttcttg acttccttgt aacctttggc ttcgagaaag
      661 tcaattgggt tcttctcgaa ggagctgcgc tccataatgg tgatacccag cagttccttc
      721 acgctcttga gcttcttaga ctttcctttt tccaccttag ccacgaccag gacagaataa
      781 gccacagttg gtgaatcgaa tccgccgtac ttcttagggt cccaatcctt cttccttgca
      841 atgagcttgt cggagttgcg cttggggagg attgattcct tagaaaagcc tccggtttgc
      901 ac
//
