LOCUS       sequence-68906-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS 
            controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcgcggata agtttgtcgt tttcgtcgta cttggtattc atgcgtgagt caaggatttg
       61 ggctacatgt ttagtaatct gccgagtctc cacaagttgc cgtttaatga atcctgcttt
      121 gtccagttcg ctcagtccgc ccctctcagc cttagtgaga ttgtcgaact tccgctgagt
      181 gatcagcttt gcgttcagaa gctggcgcca atagttcttc atctttttca cgacttcctc
      241 tgagggcacg ttatctgact ttccccggtt cttgtctgag cgagtcagca ccttattgtc
      301 aattgagtcg tctttgagga agctctgagg gacgatggcg tccacgtcgt agtctgagag
      361 cctgttgata tcaagctctt ggtccacata catatcgcgt ccattttgaa ggtagtacag
      421 atagagcttc tcattctgaa gctgggtgtt ttccaccggg tgctctttaa ggatctgtga
      481 cccgagctcc ttaatccctt cctcaattct tttcatccgc tccctgctgt tcttctgtcc
      541 cttctgggta gtctggtttt ccctggccat ctcgatgaca atgttctctg gtttatggcg
      601 acccatcacc ttgaccagct cgtccacgac tttcacggtc tggagaatac ccttcttaat
      661 agcgggtgaa ccagccagat ttgcgatatg ctcgtgcagt gagtcacctt gtccagacac
      721 ttgggccttc tggatgtcct ccttaaaggt cagggaatca tcatggataa gttgcatgaa
      781 gttgcgatta gcgaatccgt cgctcttaag gaaatcaaga atggtctttc cgctctgttt
      841 gtcccggatt ccgttgatga gtttgcggct aaggcgtccc catccggtat atcttctccg
      901 tttaagttgc ttcatcact
//
