LOCUS       sequence-68907-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS 
            controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgtcgttaag gccgtttctg acagagtaaa attcttgagg gaactttcac cattatggga
       61 aatggttcaa gaaggtattg acttaaactc catcaaatgg tcaggtcatt gagtgttttt
      121 tatttgttgt attttttttt ttttagagaa aatcctccaa tatataaatt aggaatcata
      181 gtttcatgat tttctgttac acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt
      241 aatgttaaag tgcaattctt tttccttatc acgttgagcc attagtatca atttgcttac
      301 ctgtattcct ttacatcctc ctttttctcc ttcttgataa atgtatgtag attgcgtata
      361 tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgtt tctattatga
      421 atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg
      481 attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct
      541 aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta
      601 ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg
      661 atagggttga ccttattctt tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa
      721 ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag
      781 gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt
      841 ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat
      901 tga
//
