LOCUS       sequence-68908-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and 
            Mxi1 domain controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9-Mxi1
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..174)
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     CDS             complement(208..228)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttatttaata ataaaaatca taaatcataa gaaattcgcc tcgagttagc ctctgggaga
       61 gggcatgcta gggaaagaac tggcataccc gtgttcgcat tctctttctc ttctttctaa
      121 aaactctgcg gcttctaaca gcctttgcac attaatcatt ctcacacgtt ccatgctgcc
      181 gccgctgccg ccaagcttgg aactacctac cttgcgcttt ttcttgggag ctccctcatc
      241 ccctccgagc tgtgagaggt caatccgagt ttcgtaaagt ccggtgatgc tctggtggat
      301 gagggtggcg tccagcactt ctttggtgga ggtatagcgt ttccggtcga tggtggtgtc
      361 gaagtacttg aatgcggcag gggcaccaag attggtgagg gtgaacaggt ggatgatatt
      421 ctcagcctgc tcccgaattg gtttgtctct gtgcttattg tatgcgctaa ggactttatc
      481 gaggttggca tcagcgagaa tgacgcgttt agaaaactcg gaaatctgct caatgatctc
      541 gtccagataa tgcttatgct gctccacaaa cagttgcttt tgctcgttgt cctcggggct
      601 ccccttcagc ttctcgtaat ggcttgcaag gtagaggaaa ttcacgtact tggaggggag
      661 agccagctca ttacccttct gaagttcgcc agcggaggcg agcatccgct tccgcccatt
      721 ctccagttcg aacaggctat actttgggag cttgatgata aggtccttct tgacttcctt
      781 gtaacctttg gcttcgagaa agtcaattgg gttcttctcg aaggagctgc gctccataat
      841 ggtgataccc agcagttcct tcacgctctt gagcttctta gactttcctt tt
//
