LOCUS       sequence-68919-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ350 - MCS(ttTi5605, II)
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(167..183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(190..214)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagagggtac cagagctcac ctaggtatct agaaccggtg acgtcaccat gggaagcttc
       61 gtggatccag atatcctgca ggaattcctc gagacgtacg gtgcgcgcga tgcattcgaa
      121 gatctgccca ctagtgagtc gtattacgta gcttggcgta atcatggtca tagctgtttc
      181 ctgcccgtga cccagctttc ttgtacaaag tggtaagtgc aagtaagatc agtgtttgtt
      241 tcgatatcag tctgtttcgt aacggtcttc tgtataacta caaaaaagaa taaaaaaccg
      301 tatcacaact gagaaaaaag acaaaactta ttgaacagtg ttcctttcaa aaaattattt
      361 tgtaaaaaat cgagtttttc tccaaaataa cgcacgcaat caaaaacaag aaaactaacg
      421 tgttctcctg ggcgtggaac tccttatcaa gattaccatt attgtctgtc agaattgata
      481 tttcaattat ttcatatcta gacaaacccg ggtgtcttgc atttattagt actgtgagtt
      541 tacatacaaa cgaggcttac tcagataaca tagagtcacc tctaattagc aaaaacccat
      601 ttctaattag ctatttcgac cagttctaaa gtcaccgtaa ttagaaatgg tttttgtagc
      661 ttttctaatt agaaatgacc ccgagataag tgaaatatgg cattttctgg gttttctagt
      721 aagaaatgat cccgcacgta gtgattccgc gttacaagcc aaattcaaat taaaaaagta
      781 tattacacaa ttcaagttac attattttca ggaaacaatg gcgaagagca aagcagagat
      841 t
//
