LOCUS       sequence-68920-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ355 - MCS(ttTi14024, X)
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(167..183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(190..214)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagagggtac cagagctcac ctaggtatct agaaccggtg acgtcaccat gggaagcttc
       61 gtggatccag atatcctgca ggaattcctc gagacgtacg gtgcgcgcga tgcattcgaa
      121 gatctgccca ctagtgagtc gtattacgta gcttggcgta atcatggtca tagctgtttc
      181 ctgcccgtga cccagctttc ttgtacaaag tggtttgttt gtgagtttca ggaaacttgt
      241 gaattcgttc aaacccttca gaatcagaat aaaaaatggt gctttgtatt tcttaaattt
      301 taataacttt ttcacgagca ctacatttta aaattttgta gaagttgtct gtttgcaccc
      361 atttaatttc aaatgtgtca taagtcaaca actggcacgt tgagtataca tagaaaagaa
      421 tgattttacc ttttaatatt ttttttgtta taaaagcttg caacattttt attacctcta
      481 tggataaaaa acgaaggcta ggagaaagaa tctaaaaact cggattagtt tttggataca
      541 aaacagaaga ttagaatatt tcccttcacg aattttgagg gtaaaacaat tttaccgaag
      601 ttttcaaagg tttttgttca agattccatt catttttttt gaaatacttc atacacgtgg
      661 tgtcaggttg ttacggtttg acttacaaat tttgcccaaa aaatgagacg tcagcacgtt
      721 tttagccatt taaatcagtt aagaactctg tgtctcaact cccgcatttt ttgtagatat
      781 ttgtagatca aaccgaaatg agacactctg acaccacgag ttcatattgt gaaactctct
//
